SEQUENCE_ID=Pick Primers as a reference
SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=25
PRIMER_TASK=generic
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603
PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621
PRIMER_INTERNAL_EXPLAIN=considered 9271, GC content failed 725, low tm 6079, high tm 542, long poly-x seq 92, ok 1833
PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36
PRIMER_LEFT_NUM_RETURNED=25
PRIMER_RIGHT_NUM_RETURNED=25
PRIMER_INTERNAL_NUM_RETURNED=25
PRIMER_PAIR_NUM_RETURNED=25
PRIMER_PAIR_0_PENALTY=1.171882
PRIMER_LEFT_0_PENALTY=0.117097
PRIMER_RIGHT_0_PENALTY=1.054785
PRIMER_INTERNAL_0_PENALTY=1.344173
PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc
PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga
PRIMER_INTERNAL_0_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=352,21
PRIMER_INTERNAL_0=307,21
PRIMER_LEFT_0_TM=60.117
PRIMER_RIGHT_0_TM=59.945
PRIMER_INTERNAL_0_TM=59.656
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.3000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=292
PRIMER_PAIR_1_PENALTY=1.389004
PRIMER_LEFT_1_PENALTY=1.344173
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_INTERNAL_1_PENALTY=1.054785
PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa
PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag
PRIMER_INTERNAL_1_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_1=307,21
PRIMER_RIGHT_1=528,20
PRIMER_INTERNAL_1=332,21
PRIMER_LEFT_1_TM=59.656
PRIMER_RIGHT_1_TM=59.955
PRIMER_INTERNAL_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=222
PRIMER_PAIR_2_PENALTY=1.461269
PRIMER_LEFT_2_PENALTY=0.117097
PRIMER_RIGHT_2_PENALTY=1.344173
PRIMER_INTERNAL_2_PENALTY=5.848837
PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat
PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa
PRIMER_INTERNAL_2_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_2=60,20
PRIMER_RIGHT_2=326,21
PRIMER_INTERNAL_2=273,24
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.656
PRIMER_INTERNAL_2_TM=58.151
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=267
PRIMER_PAIR_3_PENALTY=2.071477
PRIMER_LEFT_3_PENALTY=1.344173
PRIMER_RIGHT_3_PENALTY=0.727304
PRIMER_INTERNAL_3_PENALTY=1.054785
PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga
PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc
PRIMER_INTERNAL_3_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_3=306,21
PRIMER_RIGHT_3=531,20
PRIMER_INTERNAL_3=332,21
PRIMER_LEFT_3_TM=59.656
PRIMER_RIGHT_3_TM=59.273
PRIMER_INTERNAL_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=226
PRIMER_PAIR_4_PENALTY=2.122810
PRIMER_LEFT_4_PENALTY=1.058858
PRIMER_RIGHT_4_PENALTY=1.063952
PRIMER_INTERNAL_4_PENALTY=4.596606
PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg
PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg
PRIMER_INTERNAL_4_SEQUENCE=ggagacgattatgatttaattgca
PRIMER_LEFT_4=832,21
PRIMER_RIGHT_4=948,21
PRIMER_INTERNAL_4=893,24
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.936
PRIMER_INTERNAL_4_TM=59.403
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=10.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=117
PRIMER_PAIR_5_PENALTY=2.131001
PRIMER_LEFT_5_PENALTY=0.786828
PRIMER_RIGHT_5_PENALTY=1.344173
PRIMER_INTERNAL_5_PENALTY=5.848837
PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta
PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga
PRIMER_INTERNAL_5_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_5=59,20
PRIMER_RIGHT_5=327,21
PRIMER_INTERNAL_5=273,24
PRIMER_LEFT_5_TM=60.787
PRIMER_RIGHT_5_TM=59.656
PRIMER_INTERNAL_5_TM=58.151
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_INTERNAL_5_GC_PERCENT=37.500
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_INTERNAL_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_INTERNAL_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=269
PRIMER_PAIR_6_PENALTY=2.227098
PRIMER_LEFT_6_PENALTY=1.482841
PRIMER_RIGHT_6_PENALTY=0.744257
PRIMER_INTERNAL_6_PENALTY=3.156485
PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc
PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca
PRIMER_INTERNAL_6_SEQUENCE=tgatcataacagcaaaagcatg
PRIMER_LEFT_6=341,21
PRIMER_RIGHT_6=530,20
PRIMER_INTERNAL_6=362,22
PRIMER_LEFT_6_TM=59.517
PRIMER_RIGHT_6_TM=60.744
PRIMER_INTERNAL_6_TM=58.844
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=36.364
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_INTERNAL_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_INTERNAL_6_SELF_END=4.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=190
PRIMER_PAIR_7_PENALTY=2.282031
PRIMER_LEFT_7_PENALTY=0.044832
PRIMER_RIGHT_7_PENALTY=2.237199
PRIMER_INTERNAL_7_PENALTY=3.879062
PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc
PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg
PRIMER_INTERNAL_7_SEQUENCE=ttgttcattctgtttctggattg
PRIMER_LEFT_7=509,20
PRIMER_RIGHT_7=657,22
PRIMER_INTERNAL_7=593,23
PRIMER_LEFT_7_TM=59.955
PRIMER_RIGHT_7_TM=59.763
PRIMER_INTERNAL_7_TM=59.121
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_INTERNAL_7_GC_PERCENT=34.783
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=149
PRIMER_PAIR_8_PENALTY=2.676544
PRIMER_LEFT_8_PENALTY=0.833045
PRIMER_RIGHT_8_PENALTY=1.843499
PRIMER_INTERNAL_8_PENALTY=5.848837
PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc
PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg
PRIMER_INTERNAL_8_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_8=55,20
PRIMER_RIGHT_8=328,21
PRIMER_INTERNAL_8=273,24
PRIMER_LEFT_8_TM=59.167
PRIMER_RIGHT_8_TM=59.157
PRIMER_INTERNAL_8_TM=58.151
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_INTERNAL_8_GC_PERCENT=37.500
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_INTERNAL_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_INTERNAL_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=10.9000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=274
PRIMER_PAIR_9_PENALTY=3.041124
PRIMER_LEFT_9_PENALTY=1.029407
PRIMER_RIGHT_9_PENALTY=2.011716
PRIMER_INTERNAL_9_PENALTY=1.344173
PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc
PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg
PRIMER_INTERNAL_9_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_9=53,21
PRIMER_RIGHT_9=352,20
PRIMER_INTERNAL_9=307,21
PRIMER_LEFT_9_TM=60.029
PRIMER_RIGHT_9_TM=57.988
PRIMER_INTERNAL_9_TM=59.656
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_INTERNAL_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_INTERNAL_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_INTERNAL_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=300
PRIMER_PAIR_10_PENALTY=3.057112
PRIMER_LEFT_10_PENALTY=1.033556
PRIMER_RIGHT_10_PENALTY=2.023556
PRIMER_INTERNAL_10_PENALTY=5.848837
PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat
PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa
PRIMER_INTERNAL_10_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_10=64,20
PRIMER_RIGHT_10=323,22
PRIMER_INTERNAL_10=273,24
PRIMER_LEFT_10_TM=61.034
PRIMER_RIGHT_10_TM=59.976
PRIMER_INTERNAL_10_TM=58.151
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_INTERNAL_10_GC_PERCENT=37.500
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_INTERNAL_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_INTERNAL_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=260
PRIMER_PAIR_11_PENALTY=3.140307
PRIMER_LEFT_11_PENALTY=2.023556
PRIMER_RIGHT_11_PENALTY=1.116751
PRIMER_INTERNAL_11_PENALTY=1.054785
PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt
PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa
PRIMER_INTERNAL_11_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_11=303,22
PRIMER_RIGHT_11=522,21
PRIMER_INTERNAL_11=332,21
PRIMER_LEFT_11_TM=59.976
PRIMER_RIGHT_11_TM=60.117
PRIMER_INTERNAL_11_TM=59.945
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=38.095
PRIMER_INTERNAL_11_GC_PERCENT=38.095
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_INTERNAL_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_INTERNAL_11_SELF_END=4.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=220
PRIMER_PAIR_12_PENALTY=3.252729
PRIMER_LEFT_12_PENALTY=2.023556
PRIMER_RIGHT_12_PENALTY=1.229173
PRIMER_INTERNAL_12_PENALTY=1.054785
PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt
PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa
PRIMER_INTERNAL_12_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_12=302,22
PRIMER_RIGHT_12=522,20
PRIMER_INTERNAL_12=332,21
PRIMER_LEFT_12_TM=59.976
PRIMER_RIGHT_12_TM=58.771
PRIMER_INTERNAL_12_TM=59.945
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_INTERNAL_12_GC_PERCENT=38.095
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_INTERNAL_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_INTERNAL_12_SELF_END=4.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=221
PRIMER_PAIR_13_PENALTY=3.299314
PRIMER_LEFT_13_PENALTY=1.188029
PRIMER_RIGHT_13_PENALTY=2.111285
PRIMER_INTERNAL_13_PENALTY=3.306406
PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat
PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt
PRIMER_INTERNAL_13_SEQUENCE=ttggaggtgaatttctaggaca
PRIMER_LEFT_13=818,19
PRIMER_RIGHT_13=947,21
PRIMER_INTERNAL_13=846,22
PRIMER_LEFT_13_TM=60.188
PRIMER_RIGHT_13_TM=58.889
PRIMER_INTERNAL_13_TM=58.694
PRIMER_LEFT_13_GC_PERCENT=47.368
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_INTERNAL_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_INTERNAL_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_INTERNAL_13_SELF_END=2.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=130
PRIMER_PAIR_14_PENALTY=3.404939
PRIMER_LEFT_14_PENALTY=2.237199
PRIMER_RIGHT_14_PENALTY=1.167740
PRIMER_INTERNAL_14_PENALTY=4.438896
PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga
PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac
PRIMER_INTERNAL_14_SEQUENCE=gcaatcaaagattgttcttaccaa
PRIMER_LEFT_14=636,22
PRIMER_RIGHT_14=843,20
PRIMER_INTERNAL_14=721,24
PRIMER_LEFT_14_TM=59.763
PRIMER_RIGHT_14_TM=61.168
PRIMER_INTERNAL_14_TM=59.561
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_INTERNAL_14_GC_PERCENT=33.333
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_INTERNAL_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_INTERNAL_14_SELF_END=3.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=208
PRIMER_PAIR_15_PENALTY=3.404939
PRIMER_LEFT_15_PENALTY=2.237199
PRIMER_RIGHT_15_PENALTY=1.167740
PRIMER_INTERNAL_15_PENALTY=4.438896
PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg
PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa
PRIMER_INTERNAL_15_SEQUENCE=gcaatcaaagattgttcttaccaa
PRIMER_LEFT_15=635,22
PRIMER_RIGHT_15=844,20
PRIMER_INTERNAL_15=721,24
PRIMER_LEFT_15_TM=59.763
PRIMER_RIGHT_15_TM=61.168
PRIMER_INTERNAL_15_TM=59.561
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_INTERNAL_15_GC_PERCENT=33.333
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_INTERNAL_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_INTERNAL_15_SELF_END=3.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=210
PRIMER_PAIR_16_PENALTY=3.695091
PRIMER_LEFT_16_PENALTY=2.278124
PRIMER_RIGHT_16_PENALTY=1.416967
PRIMER_INTERNAL_16_PENALTY=3.541086
PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga
PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca
PRIMER_INTERNAL_16_SEQUENCE=tcataacagcaaaagcatgtg
PRIMER_LEFT_16=343,22
PRIMER_RIGHT_16=524,21
PRIMER_INTERNAL_16=365,21
PRIMER_LEFT_16_TM=59.722
PRIMER_RIGHT_16_TM=59.583
PRIMER_INTERNAL_16_TM=57.459
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_INTERNAL_16_GC_PERCENT=38.095
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_INTERNAL_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_INTERNAL_16_SELF_END=4.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=182
PRIMER_PAIR_17_PENALTY=3.707313
PRIMER_LEFT_17_PENALTY=2.290346
PRIMER_RIGHT_17_PENALTY=1.416967
PRIMER_INTERNAL_17_PENALTY=1.054785
PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg
PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc
PRIMER_INTERNAL_17_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_17=306,20
PRIMER_RIGHT_17=525,21
PRIMER_INTERNAL_17=332,21
PRIMER_LEFT_17_TM=57.710
PRIMER_RIGHT_17_TM=59.583
PRIMER_INTERNAL_17_TM=59.945
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_INTERNAL_17_GC_PERCENT=38.095
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_INTERNAL_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_INTERNAL_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=220
PRIMER_PAIR_18_PENALTY=3.755432
PRIMER_LEFT_18_PENALTY=0.727304
PRIMER_RIGHT_18_PENALTY=3.028128
PRIMER_INTERNAL_18_PENALTY=3.879062
PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt
PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat
PRIMER_INTERNAL_18_SEQUENCE=ttgttcattctgtttctggattg
PRIMER_LEFT_18=512,20
PRIMER_RIGHT_18=656,23
PRIMER_INTERNAL_18=593,23
PRIMER_LEFT_18_TM=59.273
PRIMER_RIGHT_18_TM=60.028
PRIMER_INTERNAL_18_TM=59.121
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_INTERNAL_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_INTERNAL_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_INTERNAL_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=145
PRIMER_PAIR_19_PENALTY=3.784911
PRIMER_LEFT_19_PENALTY=2.367944
PRIMER_RIGHT_19_PENALTY=1.416967
PRIMER_INTERNAL_19_PENALTY=1.054785
PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct
PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa
PRIMER_INTERNAL_19_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_19=302,21
PRIMER_RIGHT_19=527,21
PRIMER_INTERNAL_19=332,21
PRIMER_LEFT_19_TM=58.632
PRIMER_RIGHT_19_TM=59.583
PRIMER_INTERNAL_19_TM=59.945
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_INTERNAL_19_GC_PERCENT=38.095
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_INTERNAL_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_INTERNAL_19_SELF_END=4.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=226
PRIMER_PAIR_20_PENALTY=3.830133
PRIMER_LEFT_20_PENALTY=1.806577
PRIMER_RIGHT_20_PENALTY=2.023556
PRIMER_INTERNAL_20_PENALTY=5.848837
PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa
PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa
PRIMER_INTERNAL_20_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_20=64,19
PRIMER_RIGHT_20=324,22
PRIMER_INTERNAL_20=273,24
PRIMER_LEFT_20_TM=60.807
PRIMER_RIGHT_20_TM=59.976
PRIMER_INTERNAL_20_TM=58.151
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_INTERNAL_20_GC_PERCENT=37.500
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_INTERNAL_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_INTERNAL_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=261
PRIMER_PAIR_21_PENALTY=4.087459
PRIMER_LEFT_21_PENALTY=1.806597
PRIMER_RIGHT_21_PENALTY=2.280862
PRIMER_INTERNAL_21_PENALTY=1.344173
PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt
PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt
PRIMER_INTERNAL_21_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_21=59,19
PRIMER_RIGHT_21=356,22
PRIMER_INTERNAL_21=307,21
PRIMER_LEFT_21_TM=60.807
PRIMER_RIGHT_21_TM=59.719
PRIMER_INTERNAL_21_TM=59.656
PRIMER_LEFT_21_GC_PERCENT=52.632
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_INTERNAL_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=8.00
PRIMER_RIGHT_21_SELF_ANY=8.00
PRIMER_INTERNAL_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=2.00
PRIMER_RIGHT_21_SELF_END=2.00
PRIMER_INTERNAL_21_SELF_END=3.00
PRIMER_LEFT_21_END_STABILITY=11.1000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=298
PRIMER_PAIR_22_PENALTY=4.106176
PRIMER_LEFT_22_PENALTY=0.744257
PRIMER_RIGHT_22_PENALTY=3.361920
PRIMER_INTERNAL_22_PENALTY=3.879062
PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat
PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc
PRIMER_INTERNAL_22_SEQUENCE=ttgttcattctgtttctggattg
PRIMER_LEFT_22=511,20
PRIMER_RIGHT_22=654,23
PRIMER_INTERNAL_22=593,23
PRIMER_LEFT_22_TM=60.744
PRIMER_RIGHT_22_TM=59.638
PRIMER_INTERNAL_22_TM=59.121
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=39.130
PRIMER_INTERNAL_22_GC_PERCENT=34.783
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_INTERNAL_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=2.00
PRIMER_RIGHT_22_SELF_END=3.00
PRIMER_INTERNAL_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=144
PRIMER_PAIR_23_PENALTY=4.194881
PRIMER_LEFT_23_PENALTY=2.367944
PRIMER_RIGHT_23_PENALTY=1.826937
PRIMER_INTERNAL_23_PENALTY=1.054785
PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc
PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga
PRIMER_INTERNAL_23_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_23=301,21
PRIMER_RIGHT_23=527,20
PRIMER_INTERNAL_23=332,21
PRIMER_LEFT_23_TM=58.632
PRIMER_RIGHT_23_TM=58.173
PRIMER_INTERNAL_23_TM=59.945
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_INTERNAL_23_GC_PERCENT=38.095
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_INTERNAL_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_INTERNAL_23_SELF_END=4.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=227
PRIMER_PAIR_24_PENALTY=4.216158
PRIMER_LEFT_24_PENALTY=3.028128
PRIMER_RIGHT_24_PENALTY=1.188029
PRIMER_INTERNAL_24_PENALTY=4.438896
PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat
PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat
PRIMER_INTERNAL_24_SEQUENCE=gcaatcaaagattgttcttaccaa
PRIMER_LEFT_24=636,23
PRIMER_RIGHT_24=836,19
PRIMER_INTERNAL_24=721,24
PRIMER_LEFT_24_TM=60.028
PRIMER_RIGHT_24_TM=60.188
PRIMER_INTERNAL_24_TM=59.561
PRIMER_LEFT_24_GC_PERCENT=39.130
PRIMER_RIGHT_24_GC_PERCENT=47.368
PRIMER_INTERNAL_24_GC_PERCENT=33.333
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_INTERNAL_24_SELF_ANY=7.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=2.00
PRIMER_INTERNAL_24_SELF_END=3.00
PRIMER_LEFT_24_END_STABILITY=6.3000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=201
=
SEQUENCE_ID=Must obtain same result as test before
SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=25
PRIMER_TASK=generic
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
PRIMER_MUST_MATCH_THREE_PRIME=nnnnn
PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn
PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=nnnnn
PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=nnnnn
PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603
PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621
PRIMER_INTERNAL_EXPLAIN=considered 9271, GC content failed 725, low tm 6079, high tm 542, long poly-x seq 92, ok 1833
PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36
PRIMER_LEFT_NUM_RETURNED=25
PRIMER_RIGHT_NUM_RETURNED=25
PRIMER_INTERNAL_NUM_RETURNED=25
PRIMER_PAIR_NUM_RETURNED=25
PRIMER_PAIR_0_PENALTY=1.171882
PRIMER_LEFT_0_PENALTY=0.117097
PRIMER_RIGHT_0_PENALTY=1.054785
PRIMER_INTERNAL_0_PENALTY=1.344173
PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc
PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga
PRIMER_INTERNAL_0_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=352,21
PRIMER_INTERNAL_0=307,21
PRIMER_LEFT_0_TM=60.117
PRIMER_RIGHT_0_TM=59.945
PRIMER_INTERNAL_0_TM=59.656
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.3000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=292
PRIMER_PAIR_1_PENALTY=1.389004
PRIMER_LEFT_1_PENALTY=1.344173
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_INTERNAL_1_PENALTY=1.054785
PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa
PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag
PRIMER_INTERNAL_1_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_1=307,21
PRIMER_RIGHT_1=528,20
PRIMER_INTERNAL_1=332,21
PRIMER_LEFT_1_TM=59.656
PRIMER_RIGHT_1_TM=59.955
PRIMER_INTERNAL_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=222
PRIMER_PAIR_2_PENALTY=1.461269
PRIMER_LEFT_2_PENALTY=0.117097
PRIMER_RIGHT_2_PENALTY=1.344173
PRIMER_INTERNAL_2_PENALTY=5.848837
PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat
PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa
PRIMER_INTERNAL_2_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_2=60,20
PRIMER_RIGHT_2=326,21
PRIMER_INTERNAL_2=273,24
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.656
PRIMER_INTERNAL_2_TM=58.151
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=267
PRIMER_PAIR_3_PENALTY=2.071477
PRIMER_LEFT_3_PENALTY=1.344173
PRIMER_RIGHT_3_PENALTY=0.727304
PRIMER_INTERNAL_3_PENALTY=1.054785
PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga
PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc
PRIMER_INTERNAL_3_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_3=306,21
PRIMER_RIGHT_3=531,20
PRIMER_INTERNAL_3=332,21
PRIMER_LEFT_3_TM=59.656
PRIMER_RIGHT_3_TM=59.273
PRIMER_INTERNAL_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=226
PRIMER_PAIR_4_PENALTY=2.122810
PRIMER_LEFT_4_PENALTY=1.058858
PRIMER_RIGHT_4_PENALTY=1.063952
PRIMER_INTERNAL_4_PENALTY=4.596606
PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg
PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg
PRIMER_INTERNAL_4_SEQUENCE=ggagacgattatgatttaattgca
PRIMER_LEFT_4=832,21
PRIMER_RIGHT_4=948,21
PRIMER_INTERNAL_4=893,24
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.936
PRIMER_INTERNAL_4_TM=59.403
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=5.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=10.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=117
PRIMER_PAIR_5_PENALTY=2.131001
PRIMER_LEFT_5_PENALTY=0.786828
PRIMER_RIGHT_5_PENALTY=1.344173
PRIMER_INTERNAL_5_PENALTY=5.848837
PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta
PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga
PRIMER_INTERNAL_5_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_5=59,20
PRIMER_RIGHT_5=327,21
PRIMER_INTERNAL_5=273,24
PRIMER_LEFT_5_TM=60.787
PRIMER_RIGHT_5_TM=59.656
PRIMER_INTERNAL_5_TM=58.151
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_INTERNAL_5_GC_PERCENT=37.500
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_INTERNAL_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_INTERNAL_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=269
PRIMER_PAIR_6_PENALTY=2.227098
PRIMER_LEFT_6_PENALTY=1.482841
PRIMER_RIGHT_6_PENALTY=0.744257
PRIMER_INTERNAL_6_PENALTY=3.156485
PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc
PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca
PRIMER_INTERNAL_6_SEQUENCE=tgatcataacagcaaaagcatg
PRIMER_LEFT_6=341,21
PRIMER_RIGHT_6=530,20
PRIMER_INTERNAL_6=362,22
PRIMER_LEFT_6_TM=59.517
PRIMER_RIGHT_6_TM=60.744
PRIMER_INTERNAL_6_TM=58.844
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=36.364
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_INTERNAL_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_INTERNAL_6_SELF_END=4.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=190
PRIMER_PAIR_7_PENALTY=2.282031
PRIMER_LEFT_7_PENALTY=0.044832
PRIMER_RIGHT_7_PENALTY=2.237199
PRIMER_INTERNAL_7_PENALTY=3.879062
PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc
PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg
PRIMER_INTERNAL_7_SEQUENCE=ttgttcattctgtttctggattg
PRIMER_LEFT_7=509,20
PRIMER_RIGHT_7=657,22
PRIMER_INTERNAL_7=593,23
PRIMER_LEFT_7_TM=59.955
PRIMER_RIGHT_7_TM=59.763
PRIMER_INTERNAL_7_TM=59.121
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_INTERNAL_7_GC_PERCENT=34.783
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=149
PRIMER_PAIR_8_PENALTY=2.676544
PRIMER_LEFT_8_PENALTY=0.833045
PRIMER_RIGHT_8_PENALTY=1.843499
PRIMER_INTERNAL_8_PENALTY=5.848837
PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc
PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg
PRIMER_INTERNAL_8_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_8=55,20
PRIMER_RIGHT_8=328,21
PRIMER_INTERNAL_8=273,24
PRIMER_LEFT_8_TM=59.167
PRIMER_RIGHT_8_TM=59.157
PRIMER_INTERNAL_8_TM=58.151
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_INTERNAL_8_GC_PERCENT=37.500
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_INTERNAL_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_INTERNAL_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=10.9000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=274
PRIMER_PAIR_9_PENALTY=3.041124
PRIMER_LEFT_9_PENALTY=1.029407
PRIMER_RIGHT_9_PENALTY=2.011716
PRIMER_INTERNAL_9_PENALTY=1.344173
PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc
PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg
PRIMER_INTERNAL_9_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_9=53,21
PRIMER_RIGHT_9=352,20
PRIMER_INTERNAL_9=307,21
PRIMER_LEFT_9_TM=60.029
PRIMER_RIGHT_9_TM=57.988
PRIMER_INTERNAL_9_TM=59.656
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_INTERNAL_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_INTERNAL_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_INTERNAL_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=300
PRIMER_PAIR_10_PENALTY=3.057112
PRIMER_LEFT_10_PENALTY=1.033556
PRIMER_RIGHT_10_PENALTY=2.023556
PRIMER_INTERNAL_10_PENALTY=5.848837
PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat
PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa
PRIMER_INTERNAL_10_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_10=64,20
PRIMER_RIGHT_10=323,22
PRIMER_INTERNAL_10=273,24
PRIMER_LEFT_10_TM=61.034
PRIMER_RIGHT_10_TM=59.976
PRIMER_INTERNAL_10_TM=58.151
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_INTERNAL_10_GC_PERCENT=37.500
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_INTERNAL_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_INTERNAL_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=260
PRIMER_PAIR_11_PENALTY=3.140307
PRIMER_LEFT_11_PENALTY=2.023556
PRIMER_RIGHT_11_PENALTY=1.116751
PRIMER_INTERNAL_11_PENALTY=1.054785
PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt
PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa
PRIMER_INTERNAL_11_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_11=303,22
PRIMER_RIGHT_11=522,21
PRIMER_INTERNAL_11=332,21
PRIMER_LEFT_11_TM=59.976
PRIMER_RIGHT_11_TM=60.117
PRIMER_INTERNAL_11_TM=59.945
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=38.095
PRIMER_INTERNAL_11_GC_PERCENT=38.095
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_INTERNAL_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_INTERNAL_11_SELF_END=4.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=220
PRIMER_PAIR_12_PENALTY=3.252729
PRIMER_LEFT_12_PENALTY=2.023556
PRIMER_RIGHT_12_PENALTY=1.229173
PRIMER_INTERNAL_12_PENALTY=1.054785
PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt
PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa
PRIMER_INTERNAL_12_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_12=302,22
PRIMER_RIGHT_12=522,20
PRIMER_INTERNAL_12=332,21
PRIMER_LEFT_12_TM=59.976
PRIMER_RIGHT_12_TM=58.771
PRIMER_INTERNAL_12_TM=59.945
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_INTERNAL_12_GC_PERCENT=38.095
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_INTERNAL_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_INTERNAL_12_SELF_END=4.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=221
PRIMER_PAIR_13_PENALTY=3.299314
PRIMER_LEFT_13_PENALTY=1.188029
PRIMER_RIGHT_13_PENALTY=2.111285
PRIMER_INTERNAL_13_PENALTY=3.306406
PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat
PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt
PRIMER_INTERNAL_13_SEQUENCE=ttggaggtgaatttctaggaca
PRIMER_LEFT_13=818,19
PRIMER_RIGHT_13=947,21
PRIMER_INTERNAL_13=846,22
PRIMER_LEFT_13_TM=60.188
PRIMER_RIGHT_13_TM=58.889
PRIMER_INTERNAL_13_TM=58.694
PRIMER_LEFT_13_GC_PERCENT=47.368
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_INTERNAL_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_INTERNAL_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_INTERNAL_13_SELF_END=2.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=130
PRIMER_PAIR_14_PENALTY=3.404939
PRIMER_LEFT_14_PENALTY=2.237199
PRIMER_RIGHT_14_PENALTY=1.167740
PRIMER_INTERNAL_14_PENALTY=4.438896
PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga
PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac
PRIMER_INTERNAL_14_SEQUENCE=gcaatcaaagattgttcttaccaa
PRIMER_LEFT_14=636,22
PRIMER_RIGHT_14=843,20
PRIMER_INTERNAL_14=721,24
PRIMER_LEFT_14_TM=59.763
PRIMER_RIGHT_14_TM=61.168
PRIMER_INTERNAL_14_TM=59.561
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_INTERNAL_14_GC_PERCENT=33.333
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_INTERNAL_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_INTERNAL_14_SELF_END=3.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=208
PRIMER_PAIR_15_PENALTY=3.404939
PRIMER_LEFT_15_PENALTY=2.237199
PRIMER_RIGHT_15_PENALTY=1.167740
PRIMER_INTERNAL_15_PENALTY=4.438896
PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg
PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa
PRIMER_INTERNAL_15_SEQUENCE=gcaatcaaagattgttcttaccaa
PRIMER_LEFT_15=635,22
PRIMER_RIGHT_15=844,20
PRIMER_INTERNAL_15=721,24
PRIMER_LEFT_15_TM=59.763
PRIMER_RIGHT_15_TM=61.168
PRIMER_INTERNAL_15_TM=59.561
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_INTERNAL_15_GC_PERCENT=33.333
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_INTERNAL_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_INTERNAL_15_SELF_END=3.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=210
PRIMER_PAIR_16_PENALTY=3.695091
PRIMER_LEFT_16_PENALTY=2.278124
PRIMER_RIGHT_16_PENALTY=1.416967
PRIMER_INTERNAL_16_PENALTY=3.541086
PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga
PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca
PRIMER_INTERNAL_16_SEQUENCE=tcataacagcaaaagcatgtg
PRIMER_LEFT_16=343,22
PRIMER_RIGHT_16=524,21
PRIMER_INTERNAL_16=365,21
PRIMER_LEFT_16_TM=59.722
PRIMER_RIGHT_16_TM=59.583
PRIMER_INTERNAL_16_TM=57.459
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_INTERNAL_16_GC_PERCENT=38.095
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_INTERNAL_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_INTERNAL_16_SELF_END=4.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=182
PRIMER_PAIR_17_PENALTY=3.707313
PRIMER_LEFT_17_PENALTY=2.290346
PRIMER_RIGHT_17_PENALTY=1.416967
PRIMER_INTERNAL_17_PENALTY=1.054785
PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg
PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc
PRIMER_INTERNAL_17_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_17=306,20
PRIMER_RIGHT_17=525,21
PRIMER_INTERNAL_17=332,21
PRIMER_LEFT_17_TM=57.710
PRIMER_RIGHT_17_TM=59.583
PRIMER_INTERNAL_17_TM=59.945
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_INTERNAL_17_GC_PERCENT=38.095
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_INTERNAL_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_INTERNAL_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=220
PRIMER_PAIR_18_PENALTY=3.755432
PRIMER_LEFT_18_PENALTY=0.727304
PRIMER_RIGHT_18_PENALTY=3.028128
PRIMER_INTERNAL_18_PENALTY=3.879062
PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt
PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat
PRIMER_INTERNAL_18_SEQUENCE=ttgttcattctgtttctggattg
PRIMER_LEFT_18=512,20
PRIMER_RIGHT_18=656,23
PRIMER_INTERNAL_18=593,23
PRIMER_LEFT_18_TM=59.273
PRIMER_RIGHT_18_TM=60.028
PRIMER_INTERNAL_18_TM=59.121
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_INTERNAL_18_GC_PERCENT=34.783
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_INTERNAL_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_INTERNAL_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=145
PRIMER_PAIR_19_PENALTY=3.784911
PRIMER_LEFT_19_PENALTY=2.367944
PRIMER_RIGHT_19_PENALTY=1.416967
PRIMER_INTERNAL_19_PENALTY=1.054785
PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct
PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa
PRIMER_INTERNAL_19_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_19=302,21
PRIMER_RIGHT_19=527,21
PRIMER_INTERNAL_19=332,21
PRIMER_LEFT_19_TM=58.632
PRIMER_RIGHT_19_TM=59.583
PRIMER_INTERNAL_19_TM=59.945
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_INTERNAL_19_GC_PERCENT=38.095
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_INTERNAL_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_INTERNAL_19_SELF_END=4.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=226
PRIMER_PAIR_20_PENALTY=3.830133
PRIMER_LEFT_20_PENALTY=1.806577
PRIMER_RIGHT_20_PENALTY=2.023556
PRIMER_INTERNAL_20_PENALTY=5.848837
PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa
PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa
PRIMER_INTERNAL_20_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_20=64,19
PRIMER_RIGHT_20=324,22
PRIMER_INTERNAL_20=273,24
PRIMER_LEFT_20_TM=60.807
PRIMER_RIGHT_20_TM=59.976
PRIMER_INTERNAL_20_TM=58.151
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_INTERNAL_20_GC_PERCENT=37.500
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_INTERNAL_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_INTERNAL_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=261
PRIMER_PAIR_21_PENALTY=4.087459
PRIMER_LEFT_21_PENALTY=1.806597
PRIMER_RIGHT_21_PENALTY=2.280862
PRIMER_INTERNAL_21_PENALTY=1.344173
PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt
PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt
PRIMER_INTERNAL_21_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_21=59,19
PRIMER_RIGHT_21=356,22
PRIMER_INTERNAL_21=307,21
PRIMER_LEFT_21_TM=60.807
PRIMER_RIGHT_21_TM=59.719
PRIMER_INTERNAL_21_TM=59.656
PRIMER_LEFT_21_GC_PERCENT=52.632
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_INTERNAL_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=8.00
PRIMER_RIGHT_21_SELF_ANY=8.00
PRIMER_INTERNAL_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=2.00
PRIMER_RIGHT_21_SELF_END=2.00
PRIMER_INTERNAL_21_SELF_END=3.00
PRIMER_LEFT_21_END_STABILITY=11.1000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=298
PRIMER_PAIR_22_PENALTY=4.106176
PRIMER_LEFT_22_PENALTY=0.744257
PRIMER_RIGHT_22_PENALTY=3.361920
PRIMER_INTERNAL_22_PENALTY=3.879062
PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat
PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc
PRIMER_INTERNAL_22_SEQUENCE=ttgttcattctgtttctggattg
PRIMER_LEFT_22=511,20
PRIMER_RIGHT_22=654,23
PRIMER_INTERNAL_22=593,23
PRIMER_LEFT_22_TM=60.744
PRIMER_RIGHT_22_TM=59.638
PRIMER_INTERNAL_22_TM=59.121
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=39.130
PRIMER_INTERNAL_22_GC_PERCENT=34.783
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_INTERNAL_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=2.00
PRIMER_RIGHT_22_SELF_END=3.00
PRIMER_INTERNAL_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=144
PRIMER_PAIR_23_PENALTY=4.194881
PRIMER_LEFT_23_PENALTY=2.367944
PRIMER_RIGHT_23_PENALTY=1.826937
PRIMER_INTERNAL_23_PENALTY=1.054785
PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc
PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga
PRIMER_INTERNAL_23_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_23=301,21
PRIMER_RIGHT_23=527,20
PRIMER_INTERNAL_23=332,21
PRIMER_LEFT_23_TM=58.632
PRIMER_RIGHT_23_TM=58.173
PRIMER_INTERNAL_23_TM=59.945
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_INTERNAL_23_GC_PERCENT=38.095
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_INTERNAL_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_INTERNAL_23_SELF_END=4.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=227
PRIMER_PAIR_24_PENALTY=4.216158
PRIMER_LEFT_24_PENALTY=3.028128
PRIMER_RIGHT_24_PENALTY=1.188029
PRIMER_INTERNAL_24_PENALTY=4.438896
PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat
PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat
PRIMER_INTERNAL_24_SEQUENCE=gcaatcaaagattgttcttaccaa
PRIMER_LEFT_24=636,23
PRIMER_RIGHT_24=836,19
PRIMER_INTERNAL_24=721,24
PRIMER_LEFT_24_TM=60.028
PRIMER_RIGHT_24_TM=60.188
PRIMER_INTERNAL_24_TM=59.561
PRIMER_LEFT_24_GC_PERCENT=39.130
PRIMER_RIGHT_24_GC_PERCENT=47.368
PRIMER_INTERNAL_24_GC_PERCENT=33.333
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_INTERNAL_24_SELF_ANY=7.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=2.00
PRIMER_INTERNAL_24_SELF_END=3.00
PRIMER_LEFT_24_END_STABILITY=6.3000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=201
=
SEQUENCE_ID=Pick probes without G at the 3 and 5 prime end
SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=25
PRIMER_TASK=generic
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
PRIMER_MUST_MATCH_THREE_PRIME=nnnnn
PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn
PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=nnnnh
PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=hnnnn
PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603
PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621
PRIMER_INTERNAL_EXPLAIN=considered 9376, GC content failed 675, low tm 4457, high tm 370, long poly-x seq 75, failed must_match requirements 2500, ok 1299
PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36
PRIMER_LEFT_NUM_RETURNED=25
PRIMER_RIGHT_NUM_RETURNED=25
PRIMER_INTERNAL_NUM_RETURNED=25
PRIMER_PAIR_NUM_RETURNED=25
PRIMER_PAIR_0_PENALTY=1.171882
PRIMER_LEFT_0_PENALTY=0.117097
PRIMER_RIGHT_0_PENALTY=1.054785
PRIMER_INTERNAL_0_PENALTY=1.344173
PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc
PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga
PRIMER_INTERNAL_0_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=352,21
PRIMER_INTERNAL_0=307,21
PRIMER_LEFT_0_TM=60.117
PRIMER_RIGHT_0_TM=59.945
PRIMER_INTERNAL_0_TM=59.656
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=42.857
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=3.00
PRIMER_LEFT_0_END_STABILITY=5.3000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=292
PRIMER_PAIR_1_PENALTY=1.389004
PRIMER_LEFT_1_PENALTY=1.344173
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_INTERNAL_1_PENALTY=1.054785
PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa
PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag
PRIMER_INTERNAL_1_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_1=307,21
PRIMER_RIGHT_1=528,20
PRIMER_INTERNAL_1=332,21
PRIMER_LEFT_1_TM=59.656
PRIMER_RIGHT_1_TM=59.955
PRIMER_INTERNAL_1_TM=59.945
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=38.095
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=4.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=222
PRIMER_PAIR_2_PENALTY=1.461269
PRIMER_LEFT_2_PENALTY=0.117097
PRIMER_RIGHT_2_PENALTY=1.344173
PRIMER_INTERNAL_2_PENALTY=5.848837
PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat
PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa
PRIMER_INTERNAL_2_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_2=60,20
PRIMER_RIGHT_2=326,21
PRIMER_INTERNAL_2=273,24
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.656
PRIMER_INTERNAL_2_TM=58.151
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=37.500
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=4.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=1.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=267
PRIMER_PAIR_3_PENALTY=2.071477
PRIMER_LEFT_3_PENALTY=1.344173
PRIMER_RIGHT_3_PENALTY=0.727304
PRIMER_INTERNAL_3_PENALTY=1.054785
PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga
PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc
PRIMER_INTERNAL_3_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_3=306,21
PRIMER_RIGHT_3=531,20
PRIMER_INTERNAL_3=332,21
PRIMER_LEFT_3_TM=59.656
PRIMER_RIGHT_3_TM=59.273
PRIMER_INTERNAL_3_TM=59.945
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=38.095
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=4.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=226
PRIMER_PAIR_4_PENALTY=2.122810
PRIMER_LEFT_4_PENALTY=1.058858
PRIMER_RIGHT_4_PENALTY=1.063952
PRIMER_INTERNAL_4_PENALTY=4.967649
PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg
PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg
PRIMER_INTERNAL_4_SEQUENCE=acaacaaaagaaggagacgatt
PRIMER_LEFT_4=832,21
PRIMER_RIGHT_4=948,21
PRIMER_INTERNAL_4=881,22
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.936
PRIMER_INTERNAL_4_TM=57.032
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=36.364
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=2.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=1.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=10.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=117
PRIMER_PAIR_5_PENALTY=2.131001
PRIMER_LEFT_5_PENALTY=0.786828
PRIMER_RIGHT_5_PENALTY=1.344173
PRIMER_INTERNAL_5_PENALTY=5.848837
PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta
PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga
PRIMER_INTERNAL_5_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_5=59,20
PRIMER_RIGHT_5=327,21
PRIMER_INTERNAL_5=273,24
PRIMER_LEFT_5_TM=60.787
PRIMER_RIGHT_5_TM=59.656
PRIMER_INTERNAL_5_TM=58.151
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_INTERNAL_5_GC_PERCENT=37.500
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_INTERNAL_5_SELF_ANY=4.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_INTERNAL_5_SELF_END=1.00
PRIMER_LEFT_5_END_STABILITY=8.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=269
PRIMER_PAIR_6_PENALTY=2.227098
PRIMER_LEFT_6_PENALTY=1.482841
PRIMER_RIGHT_6_PENALTY=0.744257
PRIMER_INTERNAL_6_PENALTY=3.334070
PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc
PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca
PRIMER_INTERNAL_6_SEQUENCE=tgatcataacagcaaaagcatgt
PRIMER_LEFT_6=341,21
PRIMER_RIGHT_6=530,20
PRIMER_INTERNAL_6=362,23
PRIMER_LEFT_6_TM=59.517
PRIMER_RIGHT_6_TM=60.744
PRIMER_INTERNAL_6_TM=59.666
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=34.783
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_INTERNAL_6_SELF_ANY=6.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_INTERNAL_6_SELF_END=2.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=190
PRIMER_PAIR_7_PENALTY=2.282031
PRIMER_LEFT_7_PENALTY=0.044832
PRIMER_RIGHT_7_PENALTY=2.237199
PRIMER_INTERNAL_7_PENALTY=4.087031
PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc
PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg
PRIMER_INTERNAL_7_SEQUENCE=tcattctgtttctggattgtgttt
PRIMER_LEFT_7=509,20
PRIMER_RIGHT_7=657,22
PRIMER_INTERNAL_7=597,24
PRIMER_LEFT_7_TM=59.955
PRIMER_RIGHT_7_TM=59.763
PRIMER_INTERNAL_7_TM=59.913
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_INTERNAL_7_GC_PERCENT=33.333
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=3.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=149
PRIMER_PAIR_8_PENALTY=2.676544
PRIMER_LEFT_8_PENALTY=0.833045
PRIMER_RIGHT_8_PENALTY=1.843499
PRIMER_INTERNAL_8_PENALTY=5.848837
PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc
PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg
PRIMER_INTERNAL_8_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_8=55,20
PRIMER_RIGHT_8=328,21
PRIMER_INTERNAL_8=273,24
PRIMER_LEFT_8_TM=59.167
PRIMER_RIGHT_8_TM=59.157
PRIMER_INTERNAL_8_TM=58.151
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_INTERNAL_8_GC_PERCENT=37.500
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_INTERNAL_8_SELF_ANY=4.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_INTERNAL_8_SELF_END=1.00
PRIMER_LEFT_8_END_STABILITY=10.9000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=274
PRIMER_PAIR_9_PENALTY=3.041124
PRIMER_LEFT_9_PENALTY=1.029407
PRIMER_RIGHT_9_PENALTY=2.011716
PRIMER_INTERNAL_9_PENALTY=1.344173
PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc
PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg
PRIMER_INTERNAL_9_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_9=53,21
PRIMER_RIGHT_9=352,20
PRIMER_INTERNAL_9=307,21
PRIMER_LEFT_9_TM=60.029
PRIMER_RIGHT_9_TM=57.988
PRIMER_INTERNAL_9_TM=59.656
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_INTERNAL_9_GC_PERCENT=42.857
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_INTERNAL_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_INTERNAL_9_SELF_END=3.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=300
PRIMER_PAIR_10_PENALTY=3.057112
PRIMER_LEFT_10_PENALTY=1.033556
PRIMER_RIGHT_10_PENALTY=2.023556
PRIMER_INTERNAL_10_PENALTY=5.848837
PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat
PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa
PRIMER_INTERNAL_10_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_10=64,20
PRIMER_RIGHT_10=323,22
PRIMER_INTERNAL_10=273,24
PRIMER_LEFT_10_TM=61.034
PRIMER_RIGHT_10_TM=59.976
PRIMER_INTERNAL_10_TM=58.151
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_INTERNAL_10_GC_PERCENT=37.500
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_INTERNAL_10_SELF_ANY=4.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_INTERNAL_10_SELF_END=1.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=260
PRIMER_PAIR_11_PENALTY=3.140307
PRIMER_LEFT_11_PENALTY=2.023556
PRIMER_RIGHT_11_PENALTY=1.116751
PRIMER_INTERNAL_11_PENALTY=1.054785
PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt
PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa
PRIMER_INTERNAL_11_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_11=303,22
PRIMER_RIGHT_11=522,21
PRIMER_INTERNAL_11=332,21
PRIMER_LEFT_11_TM=59.976
PRIMER_RIGHT_11_TM=60.117
PRIMER_INTERNAL_11_TM=59.945
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=38.095
PRIMER_INTERNAL_11_GC_PERCENT=38.095
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_INTERNAL_11_SELF_ANY=4.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_INTERNAL_11_SELF_END=4.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=220
PRIMER_PAIR_12_PENALTY=3.252729
PRIMER_LEFT_12_PENALTY=2.023556
PRIMER_RIGHT_12_PENALTY=1.229173
PRIMER_INTERNAL_12_PENALTY=1.054785
PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt
PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa
PRIMER_INTERNAL_12_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_12=302,22
PRIMER_RIGHT_12=522,20
PRIMER_INTERNAL_12=332,21
PRIMER_LEFT_12_TM=59.976
PRIMER_RIGHT_12_TM=58.771
PRIMER_INTERNAL_12_TM=59.945
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_INTERNAL_12_GC_PERCENT=38.095
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_INTERNAL_12_SELF_ANY=4.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_INTERNAL_12_SELF_END=4.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=221
PRIMER_PAIR_13_PENALTY=3.299314
PRIMER_LEFT_13_PENALTY=1.188029
PRIMER_RIGHT_13_PENALTY=2.111285
PRIMER_INTERNAL_13_PENALTY=3.306406
PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat
PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt
PRIMER_INTERNAL_13_SEQUENCE=ttggaggtgaatttctaggaca
PRIMER_LEFT_13=818,19
PRIMER_RIGHT_13=947,21
PRIMER_INTERNAL_13=846,22
PRIMER_LEFT_13_TM=60.188
PRIMER_RIGHT_13_TM=58.889
PRIMER_INTERNAL_13_TM=58.694
PRIMER_LEFT_13_GC_PERCENT=47.368
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_INTERNAL_13_GC_PERCENT=40.909
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_INTERNAL_13_SELF_ANY=5.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_INTERNAL_13_SELF_END=2.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=130
PRIMER_PAIR_14_PENALTY=3.404939
PRIMER_LEFT_14_PENALTY=2.237199
PRIMER_RIGHT_14_PENALTY=1.167740
PRIMER_INTERNAL_14_PENALTY=4.688959
PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga
PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac
PRIMER_INTERNAL_14_SEQUENCE=tgcaatcaaagattgttcttacc
PRIMER_LEFT_14=636,22
PRIMER_RIGHT_14=843,20
PRIMER_INTERNAL_14=720,23
PRIMER_LEFT_14_TM=59.763
PRIMER_RIGHT_14_TM=61.168
PRIMER_INTERNAL_14_TM=58.311
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_INTERNAL_14_GC_PERCENT=34.783
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_INTERNAL_14_SELF_ANY=7.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_INTERNAL_14_SELF_END=0.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=208
PRIMER_PAIR_15_PENALTY=3.404939
PRIMER_LEFT_15_PENALTY=2.237199
PRIMER_RIGHT_15_PENALTY=1.167740
PRIMER_INTERNAL_15_PENALTY=4.688959
PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg
PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa
PRIMER_INTERNAL_15_SEQUENCE=tgcaatcaaagattgttcttacc
PRIMER_LEFT_15=635,22
PRIMER_RIGHT_15=844,20
PRIMER_INTERNAL_15=720,23
PRIMER_LEFT_15_TM=59.763
PRIMER_RIGHT_15_TM=61.168
PRIMER_INTERNAL_15_TM=58.311
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_INTERNAL_15_GC_PERCENT=34.783
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_INTERNAL_15_SELF_ANY=7.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_INTERNAL_15_SELF_END=0.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=210
PRIMER_PAIR_16_PENALTY=3.695091
PRIMER_LEFT_16_PENALTY=2.278124
PRIMER_RIGHT_16_PENALTY=1.416967
PRIMER_INTERNAL_16_PENALTY=3.627789
PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga
PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca
PRIMER_INTERNAL_16_SEQUENCE=tcataacagcaaaagcatgtgt
PRIMER_LEFT_16=343,22
PRIMER_RIGHT_16=524,21
PRIMER_INTERNAL_16=365,22
PRIMER_LEFT_16_TM=59.722
PRIMER_RIGHT_16_TM=59.583
PRIMER_INTERNAL_16_TM=58.372
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_INTERNAL_16_GC_PERCENT=36.364
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_INTERNAL_16_SELF_ANY=4.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_INTERNAL_16_SELF_END=2.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=182
PRIMER_PAIR_17_PENALTY=3.707313
PRIMER_LEFT_17_PENALTY=2.290346
PRIMER_RIGHT_17_PENALTY=1.416967
PRIMER_INTERNAL_17_PENALTY=1.054785
PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg
PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc
PRIMER_INTERNAL_17_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_17=306,20
PRIMER_RIGHT_17=525,21
PRIMER_INTERNAL_17=332,21
PRIMER_LEFT_17_TM=57.710
PRIMER_RIGHT_17_TM=59.583
PRIMER_INTERNAL_17_TM=59.945
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_INTERNAL_17_GC_PERCENT=38.095
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_INTERNAL_17_SELF_ANY=4.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_INTERNAL_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=220
PRIMER_PAIR_18_PENALTY=3.755432
PRIMER_LEFT_18_PENALTY=0.727304
PRIMER_RIGHT_18_PENALTY=3.028128
PRIMER_INTERNAL_18_PENALTY=4.087031
PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt
PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat
PRIMER_INTERNAL_18_SEQUENCE=tcattctgtttctggattgtgttt
PRIMER_LEFT_18=512,20
PRIMER_RIGHT_18=656,23
PRIMER_INTERNAL_18=597,24
PRIMER_LEFT_18_TM=59.273
PRIMER_RIGHT_18_TM=60.028
PRIMER_INTERNAL_18_TM=59.913
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_INTERNAL_18_GC_PERCENT=33.333
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_INTERNAL_18_SELF_ANY=3.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_INTERNAL_18_SELF_END=0.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=145
PRIMER_PAIR_19_PENALTY=3.784911
PRIMER_LEFT_19_PENALTY=2.367944
PRIMER_RIGHT_19_PENALTY=1.416967
PRIMER_INTERNAL_19_PENALTY=1.054785
PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct
PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa
PRIMER_INTERNAL_19_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_19=302,21
PRIMER_RIGHT_19=527,21
PRIMER_INTERNAL_19=332,21
PRIMER_LEFT_19_TM=58.632
PRIMER_RIGHT_19_TM=59.583
PRIMER_INTERNAL_19_TM=59.945
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_INTERNAL_19_GC_PERCENT=38.095
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_INTERNAL_19_SELF_ANY=4.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_INTERNAL_19_SELF_END=4.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=226
PRIMER_PAIR_20_PENALTY=3.830133
PRIMER_LEFT_20_PENALTY=1.806577
PRIMER_RIGHT_20_PENALTY=2.023556
PRIMER_INTERNAL_20_PENALTY=5.848837
PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa
PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa
PRIMER_INTERNAL_20_SEQUENCE=tgtcaactcttattctagccaaca
PRIMER_LEFT_20=64,19
PRIMER_RIGHT_20=324,22
PRIMER_INTERNAL_20=273,24
PRIMER_LEFT_20_TM=60.807
PRIMER_RIGHT_20_TM=59.976
PRIMER_INTERNAL_20_TM=58.151
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_INTERNAL_20_GC_PERCENT=37.500
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_INTERNAL_20_SELF_ANY=4.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_INTERNAL_20_SELF_END=1.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=261
PRIMER_PAIR_21_PENALTY=4.087459
PRIMER_LEFT_21_PENALTY=1.806597
PRIMER_RIGHT_21_PENALTY=2.280862
PRIMER_INTERNAL_21_PENALTY=1.344173
PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt
PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt
PRIMER_INTERNAL_21_SEQUENCE=tccctccacattctctttgaa
PRIMER_LEFT_21=59,19
PRIMER_RIGHT_21=356,22
PRIMER_INTERNAL_21=307,21
PRIMER_LEFT_21_TM=60.807
PRIMER_RIGHT_21_TM=59.719
PRIMER_INTERNAL_21_TM=59.656
PRIMER_LEFT_21_GC_PERCENT=52.632
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_INTERNAL_21_GC_PERCENT=42.857
PRIMER_LEFT_21_SELF_ANY=8.00
PRIMER_RIGHT_21_SELF_ANY=8.00
PRIMER_INTERNAL_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=2.00
PRIMER_RIGHT_21_SELF_END=2.00
PRIMER_INTERNAL_21_SELF_END=3.00
PRIMER_LEFT_21_END_STABILITY=11.1000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=298
PRIMER_PAIR_22_PENALTY=4.106176
PRIMER_LEFT_22_PENALTY=0.744257
PRIMER_RIGHT_22_PENALTY=3.361920
PRIMER_INTERNAL_22_PENALTY=4.087031
PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat
PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc
PRIMER_INTERNAL_22_SEQUENCE=tcattctgtttctggattgtgttt
PRIMER_LEFT_22=511,20
PRIMER_RIGHT_22=654,23
PRIMER_INTERNAL_22=597,24
PRIMER_LEFT_22_TM=60.744
PRIMER_RIGHT_22_TM=59.638
PRIMER_INTERNAL_22_TM=59.913
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=39.130
PRIMER_INTERNAL_22_GC_PERCENT=33.333
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_INTERNAL_22_SELF_ANY=3.00
PRIMER_LEFT_22_SELF_END=2.00
PRIMER_RIGHT_22_SELF_END=3.00
PRIMER_INTERNAL_22_SELF_END=0.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=144
PRIMER_PAIR_23_PENALTY=4.194881
PRIMER_LEFT_23_PENALTY=2.367944
PRIMER_RIGHT_23_PENALTY=1.826937
PRIMER_INTERNAL_23_PENALTY=1.054785
PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc
PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga
PRIMER_INTERNAL_23_SEQUENCE=tcaaacgtatccaatcatgca
PRIMER_LEFT_23=301,21
PRIMER_RIGHT_23=527,20
PRIMER_INTERNAL_23=332,21
PRIMER_LEFT_23_TM=58.632
PRIMER_RIGHT_23_TM=58.173
PRIMER_INTERNAL_23_TM=59.945
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_INTERNAL_23_GC_PERCENT=38.095
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_INTERNAL_23_SELF_ANY=4.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_INTERNAL_23_SELF_END=4.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=227
PRIMER_PAIR_24_PENALTY=4.216158
PRIMER_LEFT_24_PENALTY=3.028128
PRIMER_RIGHT_24_PENALTY=1.188029
PRIMER_INTERNAL_24_PENALTY=4.688959
PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat
PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat
PRIMER_INTERNAL_24_SEQUENCE=tgcaatcaaagattgttcttacc
PRIMER_LEFT_24=636,23
PRIMER_RIGHT_24=836,19
PRIMER_INTERNAL_24=720,23
PRIMER_LEFT_24_TM=60.028
PRIMER_RIGHT_24_TM=60.188
PRIMER_INTERNAL_24_TM=58.311
PRIMER_LEFT_24_GC_PERCENT=39.130
PRIMER_RIGHT_24_GC_PERCENT=47.368
PRIMER_INTERNAL_24_GC_PERCENT=34.783
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_INTERNAL_24_SELF_ANY=7.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=2.00
PRIMER_INTERNAL_24_SELF_END=0.00
PRIMER_LEFT_24_END_STABILITY=6.3000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=201
=
SEQUENCE_ID=Pick probes without G at 5 nucleotides the 3 and 5 prime end
SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=25
PRIMER_TASK=generic
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
PRIMER_MUST_MATCH_THREE_PRIME=nnnnn
PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn
PRIMER_INTERNAL_MUST_MATCH_THREE_PRIME=hhhhh
PRIMER_INTERNAL_MUST_MATCH_FIVE_PRIME=hhhhh
PRIMER_LEFT_EXPLAIN=considered 8496, GC content failed 704, low tm 5558, high tm 529, high end compl 10, long poly-x seq 92, ok 1603
PRIMER_RIGHT_EXPLAIN=considered 8494, GC content failed 723, low tm 5642, high tm 406, high end compl 10, long poly-x seq 92, ok 1621
PRIMER_INTERNAL_EXPLAIN=considered 9631, GC content failed 422, low tm 1156, high tm 51, long poly-x seq 37, failed must_match requirements 7722, ok 243
PRIMER_PAIR_EXPLAIN=considered 2657, unacceptable product size 2620, high end compl 1, primer in pair overlaps a primer in a better pair 2423, ok 36
PRIMER_LEFT_NUM_RETURNED=25
PRIMER_RIGHT_NUM_RETURNED=25
PRIMER_INTERNAL_NUM_RETURNED=25
PRIMER_PAIR_NUM_RETURNED=25
PRIMER_PAIR_0_PENALTY=1.171882
PRIMER_LEFT_0_PENALTY=0.117097
PRIMER_RIGHT_0_PENALTY=1.054785
PRIMER_INTERNAL_0_PENALTY=2.515892
PRIMER_LEFT_0_SEQUENCE=attcgattctccccggtatc
PRIMER_RIGHT_0_SEQUENCE=tgcatgattggatacgtttga
PRIMER_INTERNAL_0_SEQUENCE=actttgttccctccacattctc
PRIMER_LEFT_0=61,20
PRIMER_RIGHT_0=352,21
PRIMER_INTERNAL_0=300,22
PRIMER_LEFT_0_TM=60.117
PRIMER_RIGHT_0_TM=59.945
PRIMER_INTERNAL_0_TM=59.484
PRIMER_LEFT_0_GC_PERCENT=50.000
PRIMER_RIGHT_0_GC_PERCENT=38.095
PRIMER_INTERNAL_0_GC_PERCENT=45.455
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=4.00
PRIMER_INTERNAL_0_SELF_ANY=3.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=1.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=5.3000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=3.00
PRIMER_PAIR_0_PRODUCT_SIZE=292
PRIMER_PAIR_1_PENALTY=1.389004
PRIMER_LEFT_1_PENALTY=1.344173
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_INTERNAL_1_PENALTY=4.782051
PRIMER_LEFT_1_SEQUENCE=tccctccacattctctttgaa
PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag
PRIMER_INTERNAL_1_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_1=307,21
PRIMER_RIGHT_1=528,20
PRIMER_INTERNAL_1=345,24
PRIMER_LEFT_1_TM=59.656
PRIMER_RIGHT_1_TM=59.955
PRIMER_INTERNAL_1_TM=60.782
PRIMER_LEFT_1_GC_PERCENT=42.857
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=37.500
PRIMER_LEFT_1_SELF_ANY=3.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=8.00
PRIMER_LEFT_1_SELF_END=3.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=4.00
PRIMER_LEFT_1_END_STABILITY=7.3000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=4.00
PRIMER_PAIR_1_COMPL_END=0.00
PRIMER_PAIR_1_PRODUCT_SIZE=222
PRIMER_PAIR_2_PENALTY=1.461269
PRIMER_LEFT_2_PENALTY=0.117097
PRIMER_RIGHT_2_PENALTY=1.344173
PRIMER_INTERNAL_2_PENALTY=7.331111
PRIMER_LEFT_2_SEQUENCE=gattcgattctccccggtat
PRIMER_RIGHT_2_SEQUENCE=tcaaagagaatgtggagggaa
PRIMER_INTERNAL_2_SEQUENCE=aacatgtcaactcttattctagccaac
PRIMER_LEFT_2=60,20
PRIMER_RIGHT_2=326,21
PRIMER_INTERNAL_2=269,27
PRIMER_LEFT_2_TM=60.117
PRIMER_RIGHT_2_TM=59.656
PRIMER_INTERNAL_2_TM=60.331
PRIMER_LEFT_2_GC_PERCENT=50.000
PRIMER_RIGHT_2_GC_PERCENT=42.857
PRIMER_INTERNAL_2_GC_PERCENT=37.037
PRIMER_LEFT_2_SELF_ANY=6.00
PRIMER_RIGHT_2_SELF_ANY=2.00
PRIMER_INTERNAL_2_SELF_ANY=6.00
PRIMER_LEFT_2_SELF_END=2.00
PRIMER_RIGHT_2_SELF_END=0.00
PRIMER_INTERNAL_2_SELF_END=0.00
PRIMER_LEFT_2_END_STABILITY=6.8000
PRIMER_RIGHT_2_END_STABILITY=9.7000
PRIMER_PAIR_2_COMPL_ANY=6.00
PRIMER_PAIR_2_COMPL_END=2.00
PRIMER_PAIR_2_PRODUCT_SIZE=267
PRIMER_PAIR_3_PENALTY=2.071477
PRIMER_LEFT_3_PENALTY=1.344173
PRIMER_RIGHT_3_PENALTY=0.727304
PRIMER_INTERNAL_3_PENALTY=4.782051
PRIMER_LEFT_3_SEQUENCE=ttccctccacattctctttga
PRIMER_RIGHT_3_SEQUENCE=aatggctgatgaaggaaagc
PRIMER_INTERNAL_3_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_3=306,21
PRIMER_RIGHT_3=531,20
PRIMER_INTERNAL_3=345,24
PRIMER_LEFT_3_TM=59.656
PRIMER_RIGHT_3_TM=59.273
PRIMER_INTERNAL_3_TM=60.782
PRIMER_LEFT_3_GC_PERCENT=42.857
PRIMER_RIGHT_3_GC_PERCENT=45.000
PRIMER_INTERNAL_3_GC_PERCENT=37.500
PRIMER_LEFT_3_SELF_ANY=2.00
PRIMER_RIGHT_3_SELF_ANY=3.00
PRIMER_INTERNAL_3_SELF_ANY=8.00
PRIMER_LEFT_3_SELF_END=1.00
PRIMER_RIGHT_3_SELF_END=2.00
PRIMER_INTERNAL_3_SELF_END=4.00
PRIMER_LEFT_3_END_STABILITY=7.3000
PRIMER_RIGHT_3_END_STABILITY=8.5000
PRIMER_PAIR_3_COMPL_ANY=5.00
PRIMER_PAIR_3_COMPL_END=1.00
PRIMER_PAIR_3_PRODUCT_SIZE=226
PRIMER_PAIR_4_PENALTY=2.122810
PRIMER_LEFT_4_PENALTY=1.058858
PRIMER_RIGHT_4_PENALTY=1.063952
PRIMER_INTERNAL_4_PENALTY=6.476933
PRIMER_LEFT_4_SEQUENCE=cccatgtgaatgtattggagg
PRIMER_RIGHT_4_SEQUENCE=tcaacagttgtcaaacttgcg
PRIMER_INTERNAL_4_SEQUENCE=acaacaaaagaaggagacgattat
PRIMER_LEFT_4=832,21
PRIMER_RIGHT_4=948,21
PRIMER_INTERNAL_4=881,24
PRIMER_LEFT_4_TM=60.059
PRIMER_RIGHT_4_TM=59.936
PRIMER_INTERNAL_4_TM=57.523
PRIMER_LEFT_4_GC_PERCENT=47.619
PRIMER_RIGHT_4_GC_PERCENT=42.857
PRIMER_INTERNAL_4_GC_PERCENT=33.333
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=3.00
PRIMER_LEFT_4_SELF_END=0.00
PRIMER_RIGHT_4_SELF_END=2.00
PRIMER_INTERNAL_4_SELF_END=3.00
PRIMER_LEFT_4_END_STABILITY=9.4000
PRIMER_RIGHT_4_END_STABILITY=10.5000
PRIMER_PAIR_4_COMPL_ANY=3.00
PRIMER_PAIR_4_COMPL_END=0.00
PRIMER_PAIR_4_PRODUCT_SIZE=117
PRIMER_PAIR_5_PENALTY=2.131001
PRIMER_LEFT_5_PENALTY=0.786828
PRIMER_RIGHT_5_PENALTY=1.344173
PRIMER_INTERNAL_5_PENALTY=7.331111
PRIMER_LEFT_5_SEQUENCE=agattcgattctccccggta
PRIMER_RIGHT_5_SEQUENCE=ttcaaagagaatgtggaggga
PRIMER_INTERNAL_5_SEQUENCE=aacatgtcaactcttattctagccaac
PRIMER_LEFT_5=59,20
PRIMER_RIGHT_5=327,21
PRIMER_INTERNAL_5=269,27
PRIMER_LEFT_5_TM=60.787
PRIMER_RIGHT_5_TM=59.656
PRIMER_INTERNAL_5_TM=60.331
PRIMER_LEFT_5_GC_PERCENT=50.000
PRIMER_RIGHT_5_GC_PERCENT=42.857
PRIMER_INTERNAL_5_GC_PERCENT=37.037
PRIMER_LEFT_5_SELF_ANY=8.00
PRIMER_RIGHT_5_SELF_ANY=3.00
PRIMER_INTERNAL_5_SELF_ANY=6.00
PRIMER_LEFT_5_SELF_END=3.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_INTERNAL_5_SELF_END=0.00
PRIMER_LEFT_5_END_STABILITY=8.9000
PRIMER_RIGHT_5_END_STABILITY=9.4000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=1.00
PRIMER_PAIR_5_PRODUCT_SIZE=269
PRIMER_PAIR_6_PENALTY=2.227098
PRIMER_LEFT_6_PENALTY=1.482841
PRIMER_RIGHT_6_PENALTY=0.744257
PRIMER_INTERNAL_6_PENALTY=5.033056
PRIMER_LEFT_6_SEQUENCE=tccaatcatgcatgtctgttc
PRIMER_RIGHT_6_SEQUENCE=atggctgatgaaggaaagca
PRIMER_INTERNAL_6_SEQUENCE=tttttgctgatttgagattctttc
PRIMER_LEFT_6=341,21
PRIMER_RIGHT_6=530,20
PRIMER_INTERNAL_6=474,24
PRIMER_LEFT_6_TM=59.517
PRIMER_RIGHT_6_TM=60.744
PRIMER_INTERNAL_6_TM=58.967
PRIMER_LEFT_6_GC_PERCENT=42.857
PRIMER_RIGHT_6_GC_PERCENT=45.000
PRIMER_INTERNAL_6_GC_PERCENT=29.167
PRIMER_LEFT_6_SELF_ANY=8.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_INTERNAL_6_SELF_ANY=5.00
PRIMER_LEFT_6_SELF_END=2.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_INTERNAL_6_SELF_END=1.00
PRIMER_LEFT_6_END_STABILITY=6.7000
PRIMER_RIGHT_6_END_STABILITY=8.5000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=3.00
PRIMER_PAIR_6_PRODUCT_SIZE=190
PRIMER_PAIR_7_PENALTY=2.282031
PRIMER_LEFT_7_PENALTY=0.044832
PRIMER_RIGHT_7_PENALTY=2.237199
PRIMER_INTERNAL_7_PENALTY=5.614815
PRIMER_LEFT_7_SEQUENCE=cttgctttccttcatcagcc
PRIMER_RIGHT_7_SEQUENCE=tcacacccttttacacatttgg
PRIMER_INTERNAL_7_SEQUENCE=tctcttcttgattcaatgaatctca
PRIMER_LEFT_7=509,20
PRIMER_RIGHT_7=657,22
PRIMER_INTERNAL_7=554,25
PRIMER_LEFT_7_TM=59.955
PRIMER_RIGHT_7_TM=59.763
PRIMER_INTERNAL_7_TM=59.385
PRIMER_LEFT_7_GC_PERCENT=50.000
PRIMER_RIGHT_7_GC_PERCENT=40.909
PRIMER_INTERNAL_7_GC_PERCENT=32.000
PRIMER_LEFT_7_SELF_ANY=3.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=11.00
PRIMER_LEFT_7_SELF_END=1.00
PRIMER_RIGHT_7_SELF_END=2.00
PRIMER_INTERNAL_7_SELF_END=7.00
PRIMER_LEFT_7_END_STABILITY=9.7000
PRIMER_RIGHT_7_END_STABILITY=8.8000
PRIMER_PAIR_7_COMPL_ANY=2.00
PRIMER_PAIR_7_COMPL_END=2.00
PRIMER_PAIR_7_PRODUCT_SIZE=149
PRIMER_PAIR_8_PENALTY=2.676544
PRIMER_LEFT_8_PENALTY=0.833045
PRIMER_RIGHT_8_PENALTY=1.843499
PRIMER_INTERNAL_8_PENALTY=7.331111
PRIMER_LEFT_8_SEQUENCE=tctcagattcgattctcccc
PRIMER_RIGHT_8_SEQUENCE=tttcaaagagaatgtggaggg
PRIMER_INTERNAL_8_SEQUENCE=aacatgtcaactcttattctagccaac
PRIMER_LEFT_8=55,20
PRIMER_RIGHT_8=328,21
PRIMER_INTERNAL_8=269,27
PRIMER_LEFT_8_TM=59.167
PRIMER_RIGHT_8_TM=59.157
PRIMER_INTERNAL_8_TM=60.331
PRIMER_LEFT_8_GC_PERCENT=50.000
PRIMER_RIGHT_8_GC_PERCENT=42.857
PRIMER_INTERNAL_8_GC_PERCENT=37.037
PRIMER_LEFT_8_SELF_ANY=8.00
PRIMER_RIGHT_8_SELF_ANY=5.00
PRIMER_INTERNAL_8_SELF_ANY=6.00
PRIMER_LEFT_8_SELF_END=0.00
PRIMER_RIGHT_8_SELF_END=0.00
PRIMER_INTERNAL_8_SELF_END=0.00
PRIMER_LEFT_8_END_STABILITY=10.9000
PRIMER_RIGHT_8_END_STABILITY=9.4000
PRIMER_PAIR_8_COMPL_ANY=6.00
PRIMER_PAIR_8_COMPL_END=3.00
PRIMER_PAIR_8_PRODUCT_SIZE=274
PRIMER_PAIR_9_PENALTY=3.041124
PRIMER_LEFT_9_PENALTY=1.029407
PRIMER_RIGHT_9_PENALTY=2.011716
PRIMER_INTERNAL_9_PENALTY=2.515892
PRIMER_LEFT_9_SEQUENCE=ggtctcagattcgattctccc
PRIMER_RIGHT_9_SEQUENCE=tgcatgattggatacgtttg
PRIMER_INTERNAL_9_SEQUENCE=actttgttccctccacattctc
PRIMER_LEFT_9=53,21
PRIMER_RIGHT_9=352,20
PRIMER_INTERNAL_9=300,22
PRIMER_LEFT_9_TM=60.029
PRIMER_RIGHT_9_TM=57.988
PRIMER_INTERNAL_9_TM=59.484
PRIMER_LEFT_9_GC_PERCENT=52.381
PRIMER_RIGHT_9_GC_PERCENT=40.000
PRIMER_INTERNAL_9_GC_PERCENT=45.455
PRIMER_LEFT_9_SELF_ANY=8.00
PRIMER_RIGHT_9_SELF_ANY=4.00
PRIMER_INTERNAL_9_SELF_ANY=3.00
PRIMER_LEFT_9_SELF_END=2.00
PRIMER_RIGHT_9_SELF_END=2.00
PRIMER_INTERNAL_9_SELF_END=0.00
PRIMER_LEFT_9_END_STABILITY=9.4000
PRIMER_RIGHT_9_END_STABILITY=7.0000
PRIMER_PAIR_9_COMPL_ANY=3.00
PRIMER_PAIR_9_COMPL_END=1.00
PRIMER_PAIR_9_PRODUCT_SIZE=300
PRIMER_PAIR_10_PENALTY=3.057112
PRIMER_LEFT_10_PENALTY=1.033556
PRIMER_RIGHT_10_PENALTY=2.023556
PRIMER_INTERNAL_10_PENALTY=7.331111
PRIMER_LEFT_10_SEQUENCE=cgattctccccggtatcaat
PRIMER_RIGHT_10_SEQUENCE=aagagaatgtggagggaacaaa
PRIMER_INTERNAL_10_SEQUENCE=aacatgtcaactcttattctagccaac
PRIMER_LEFT_10=64,20
PRIMER_RIGHT_10=323,22
PRIMER_INTERNAL_10=269,27
PRIMER_LEFT_10_TM=61.034
PRIMER_RIGHT_10_TM=59.976
PRIMER_INTERNAL_10_TM=60.331
PRIMER_LEFT_10_GC_PERCENT=50.000
PRIMER_RIGHT_10_GC_PERCENT=40.909
PRIMER_INTERNAL_10_GC_PERCENT=37.037
PRIMER_LEFT_10_SELF_ANY=4.00
PRIMER_RIGHT_10_SELF_ANY=3.00
PRIMER_INTERNAL_10_SELF_ANY=6.00
PRIMER_LEFT_10_SELF_END=3.00
PRIMER_RIGHT_10_SELF_END=0.00
PRIMER_INTERNAL_10_SELF_END=0.00
PRIMER_LEFT_10_END_STABILITY=6.9000
PRIMER_RIGHT_10_END_STABILITY=7.0000
PRIMER_PAIR_10_COMPL_ANY=6.00
PRIMER_PAIR_10_COMPL_END=1.00
PRIMER_PAIR_10_PRODUCT_SIZE=260
PRIMER_PAIR_11_PENALTY=3.140307
PRIMER_LEFT_11_PENALTY=2.023556
PRIMER_RIGHT_11_PENALTY=1.116751
PRIMER_INTERNAL_11_PENALTY=4.782051
PRIMER_LEFT_11_SEQUENCE=ttgttccctccacattctcttt
PRIMER_RIGHT_11_SEQUENCE=tgaaggaaagcaagaagcaaa
PRIMER_INTERNAL_11_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_11=303,22
PRIMER_RIGHT_11=522,21
PRIMER_INTERNAL_11=345,24
PRIMER_LEFT_11_TM=59.976
PRIMER_RIGHT_11_TM=60.117
PRIMER_INTERNAL_11_TM=60.782
PRIMER_LEFT_11_GC_PERCENT=40.909
PRIMER_RIGHT_11_GC_PERCENT=38.095
PRIMER_INTERNAL_11_GC_PERCENT=37.500
PRIMER_LEFT_11_SELF_ANY=3.00
PRIMER_RIGHT_11_SELF_ANY=2.00
PRIMER_INTERNAL_11_SELF_ANY=8.00
PRIMER_LEFT_11_SELF_END=0.00
PRIMER_RIGHT_11_SELF_END=0.00
PRIMER_INTERNAL_11_SELF_END=4.00
PRIMER_LEFT_11_END_STABILITY=7.0000
PRIMER_RIGHT_11_END_STABILITY=8.8000
PRIMER_PAIR_11_COMPL_ANY=6.00
PRIMER_PAIR_11_COMPL_END=3.00
PRIMER_PAIR_11_PRODUCT_SIZE=220
PRIMER_PAIR_12_PENALTY=3.252729
PRIMER_LEFT_12_PENALTY=2.023556
PRIMER_RIGHT_12_PENALTY=1.229173
PRIMER_INTERNAL_12_PENALTY=4.782051
PRIMER_LEFT_12_SEQUENCE=tttgttccctccacattctctt
PRIMER_RIGHT_12_SEQUENCE=tgaaggaaagcaagaagcaa
PRIMER_INTERNAL_12_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_12=302,22
PRIMER_RIGHT_12=522,20
PRIMER_INTERNAL_12=345,24
PRIMER_LEFT_12_TM=59.976
PRIMER_RIGHT_12_TM=58.771
PRIMER_INTERNAL_12_TM=60.782
PRIMER_LEFT_12_GC_PERCENT=40.909
PRIMER_RIGHT_12_GC_PERCENT=40.000
PRIMER_INTERNAL_12_GC_PERCENT=37.500
PRIMER_LEFT_12_SELF_ANY=3.00
PRIMER_RIGHT_12_SELF_ANY=2.00
PRIMER_INTERNAL_12_SELF_ANY=8.00
PRIMER_LEFT_12_SELF_END=0.00
PRIMER_RIGHT_12_SELF_END=0.00
PRIMER_INTERNAL_12_SELF_END=4.00
PRIMER_LEFT_12_END_STABILITY=6.7000
PRIMER_RIGHT_12_END_STABILITY=8.5000
PRIMER_PAIR_12_COMPL_ANY=6.00
PRIMER_PAIR_12_COMPL_END=3.00
PRIMER_PAIR_12_PRODUCT_SIZE=221
PRIMER_PAIR_13_PENALTY=3.299314
PRIMER_LEFT_13_PENALTY=1.188029
PRIMER_RIGHT_13_PENALTY=2.111285
PRIMER_INTERNAL_13_PENALTY=6.476933
PRIMER_LEFT_13_SEQUENCE=atcaacgttccgttcccat
PRIMER_RIGHT_13_SEQUENCE=caacagttgtcaaacttgcgt
PRIMER_INTERNAL_13_SEQUENCE=acaacaaaagaaggagacgattat
PRIMER_LEFT_13=818,19
PRIMER_RIGHT_13=947,21
PRIMER_INTERNAL_13=881,24
PRIMER_LEFT_13_TM=60.188
PRIMER_RIGHT_13_TM=58.889
PRIMER_INTERNAL_13_TM=57.523
PRIMER_LEFT_13_GC_PERCENT=47.368
PRIMER_RIGHT_13_GC_PERCENT=42.857
PRIMER_INTERNAL_13_GC_PERCENT=33.333
PRIMER_LEFT_13_SELF_ANY=6.00
PRIMER_RIGHT_13_SELF_ANY=7.00
PRIMER_INTERNAL_13_SELF_ANY=3.00
PRIMER_LEFT_13_SELF_END=2.00
PRIMER_RIGHT_13_SELF_END=1.00
PRIMER_INTERNAL_13_SELF_END=3.00
PRIMER_LEFT_13_END_STABILITY=9.6000
PRIMER_RIGHT_13_END_STABILITY=9.9000
PRIMER_PAIR_13_COMPL_ANY=5.00
PRIMER_PAIR_13_COMPL_END=0.00
PRIMER_PAIR_13_PRODUCT_SIZE=130
PRIMER_PAIR_14_PENALTY=3.404939
PRIMER_LEFT_14_PENALTY=2.237199
PRIMER_RIGHT_14_PENALTY=1.167740
PRIMER_INTERNAL_14_PENALTY=5.389860
PRIMER_LEFT_14_SEQUENCE=ccaaatgtgtaaaagggtgtga
PRIMER_RIGHT_14_SEQUENCE=cattcacatgggaacggaac
PRIMER_INTERNAL_14_SEQUENCE=tcaaagattgttcttaccaattcc
PRIMER_LEFT_14=636,22
PRIMER_RIGHT_14=843,20
PRIMER_INTERNAL_14=725,24
PRIMER_LEFT_14_TM=59.763
PRIMER_RIGHT_14_TM=61.168
PRIMER_INTERNAL_14_TM=58.610
PRIMER_LEFT_14_GC_PERCENT=40.909
PRIMER_RIGHT_14_GC_PERCENT=50.000
PRIMER_INTERNAL_14_GC_PERCENT=33.333
PRIMER_LEFT_14_SELF_ANY=4.00
PRIMER_RIGHT_14_SELF_ANY=8.00
PRIMER_INTERNAL_14_SELF_ANY=5.00
PRIMER_LEFT_14_SELF_END=1.00
PRIMER_RIGHT_14_SELF_END=0.00
PRIMER_INTERNAL_14_SELF_END=1.00
PRIMER_LEFT_14_END_STABILITY=6.7000
PRIMER_RIGHT_14_END_STABILITY=7.9000
PRIMER_PAIR_14_COMPL_ANY=5.00
PRIMER_PAIR_14_COMPL_END=0.00
PRIMER_PAIR_14_PRODUCT_SIZE=208
PRIMER_PAIR_15_PENALTY=3.404939
PRIMER_LEFT_15_PENALTY=2.237199
PRIMER_RIGHT_15_PENALTY=1.167740
PRIMER_INTERNAL_15_PENALTY=5.389860
PRIMER_LEFT_15_SEQUENCE=tccaaatgtgtaaaagggtgtg
PRIMER_RIGHT_15_SEQUENCE=acattcacatgggaacggaa
PRIMER_INTERNAL_15_SEQUENCE=tcaaagattgttcttaccaattcc
PRIMER_LEFT_15=635,22
PRIMER_RIGHT_15=844,20
PRIMER_INTERNAL_15=725,24
PRIMER_LEFT_15_TM=59.763
PRIMER_RIGHT_15_TM=61.168
PRIMER_INTERNAL_15_TM=58.610
PRIMER_LEFT_15_GC_PERCENT=40.909
PRIMER_RIGHT_15_GC_PERCENT=45.000
PRIMER_INTERNAL_15_GC_PERCENT=33.333
PRIMER_LEFT_15_SELF_ANY=4.00
PRIMER_RIGHT_15_SELF_ANY=8.00
PRIMER_INTERNAL_15_SELF_ANY=5.00
PRIMER_LEFT_15_SELF_END=0.00
PRIMER_RIGHT_15_SELF_END=2.00
PRIMER_INTERNAL_15_SELF_END=1.00
PRIMER_LEFT_15_END_STABILITY=6.4000
PRIMER_RIGHT_15_END_STABILITY=10.2000
PRIMER_PAIR_15_COMPL_ANY=5.00
PRIMER_PAIR_15_COMPL_END=0.00
PRIMER_PAIR_15_PRODUCT_SIZE=210
PRIMER_PAIR_16_PENALTY=3.695091
PRIMER_LEFT_16_PENALTY=2.278124
PRIMER_RIGHT_16_PENALTY=1.416967
PRIMER_INTERNAL_16_PENALTY=5.033056
PRIMER_LEFT_16_SEQUENCE=caatcatgcatgtctgttctga
PRIMER_RIGHT_16_SEQUENCE=gatgaaggaaagcaagaagca
PRIMER_INTERNAL_16_SEQUENCE=tttttgctgatttgagattctttc
PRIMER_LEFT_16=343,22
PRIMER_RIGHT_16=524,21
PRIMER_INTERNAL_16=474,24
PRIMER_LEFT_16_TM=59.722
PRIMER_RIGHT_16_TM=59.583
PRIMER_INTERNAL_16_TM=58.967
PRIMER_LEFT_16_GC_PERCENT=40.909
PRIMER_RIGHT_16_GC_PERCENT=42.857
PRIMER_INTERNAL_16_GC_PERCENT=29.167
PRIMER_LEFT_16_SELF_ANY=8.00
PRIMER_RIGHT_16_SELF_ANY=2.00
PRIMER_INTERNAL_16_SELF_ANY=5.00
PRIMER_LEFT_16_SELF_END=3.00
PRIMER_RIGHT_16_SELF_END=0.00
PRIMER_INTERNAL_16_SELF_END=1.00
PRIMER_LEFT_16_END_STABILITY=6.7000
PRIMER_RIGHT_16_END_STABILITY=8.5000
PRIMER_PAIR_16_COMPL_ANY=4.00
PRIMER_PAIR_16_COMPL_END=2.00
PRIMER_PAIR_16_PRODUCT_SIZE=182
PRIMER_PAIR_17_PENALTY=3.707313
PRIMER_LEFT_17_PENALTY=2.290346
PRIMER_RIGHT_17_PENALTY=1.416967
PRIMER_INTERNAL_17_PENALTY=4.782051
PRIMER_LEFT_17_SEQUENCE=ttccctccacattctctttg
PRIMER_RIGHT_17_SEQUENCE=tgatgaaggaaagcaagaagc
PRIMER_INTERNAL_17_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_17=306,20
PRIMER_RIGHT_17=525,21
PRIMER_INTERNAL_17=345,24
PRIMER_LEFT_17_TM=57.710
PRIMER_RIGHT_17_TM=59.583
PRIMER_INTERNAL_17_TM=60.782
PRIMER_LEFT_17_GC_PERCENT=45.000
PRIMER_RIGHT_17_GC_PERCENT=42.857
PRIMER_INTERNAL_17_GC_PERCENT=37.500
PRIMER_LEFT_17_SELF_ANY=2.00
PRIMER_RIGHT_17_SELF_ANY=2.00
PRIMER_INTERNAL_17_SELF_ANY=8.00
PRIMER_LEFT_17_SELF_END=0.00
PRIMER_RIGHT_17_SELF_END=2.00
PRIMER_INTERNAL_17_SELF_END=4.00
PRIMER_LEFT_17_END_STABILITY=7.3000
PRIMER_RIGHT_17_END_STABILITY=8.2000
PRIMER_PAIR_17_COMPL_ANY=5.00
PRIMER_PAIR_17_COMPL_END=2.00
PRIMER_PAIR_17_PRODUCT_SIZE=220
PRIMER_PAIR_18_PENALTY=3.755432
PRIMER_LEFT_18_PENALTY=0.727304
PRIMER_RIGHT_18_PENALTY=3.028128
PRIMER_INTERNAL_18_PENALTY=5.614815
PRIMER_LEFT_18_SEQUENCE=gctttccttcatcagccatt
PRIMER_RIGHT_18_SEQUENCE=cacacccttttacacatttggat
PRIMER_INTERNAL_18_SEQUENCE=tctcttcttgattcaatgaatctca
PRIMER_LEFT_18=512,20
PRIMER_RIGHT_18=656,23
PRIMER_INTERNAL_18=554,25
PRIMER_LEFT_18_TM=59.273
PRIMER_RIGHT_18_TM=60.028
PRIMER_INTERNAL_18_TM=59.385
PRIMER_LEFT_18_GC_PERCENT=45.000
PRIMER_RIGHT_18_GC_PERCENT=39.130
PRIMER_INTERNAL_18_GC_PERCENT=32.000
PRIMER_LEFT_18_SELF_ANY=3.00
PRIMER_RIGHT_18_SELF_ANY=4.00
PRIMER_INTERNAL_18_SELF_ANY=11.00
PRIMER_LEFT_18_SELF_END=1.00
PRIMER_RIGHT_18_SELF_END=2.00
PRIMER_INTERNAL_18_SELF_END=7.00
PRIMER_LEFT_18_END_STABILITY=8.4000
PRIMER_RIGHT_18_END_STABILITY=8.1000
PRIMER_PAIR_18_COMPL_ANY=3.00
PRIMER_PAIR_18_COMPL_END=1.00
PRIMER_PAIR_18_PRODUCT_SIZE=145
PRIMER_PAIR_19_PENALTY=3.784911
PRIMER_LEFT_19_PENALTY=2.367944
PRIMER_RIGHT_19_PENALTY=1.416967
PRIMER_INTERNAL_19_PENALTY=4.782051
PRIMER_LEFT_19_SEQUENCE=tttgttccctccacattctct
PRIMER_RIGHT_19_SEQUENCE=gctgatgaaggaaagcaagaa
PRIMER_INTERNAL_19_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_19=302,21
PRIMER_RIGHT_19=527,21
PRIMER_INTERNAL_19=345,24
PRIMER_LEFT_19_TM=58.632
PRIMER_RIGHT_19_TM=59.583
PRIMER_INTERNAL_19_TM=60.782
PRIMER_LEFT_19_GC_PERCENT=42.857
PRIMER_RIGHT_19_GC_PERCENT=42.857
PRIMER_INTERNAL_19_GC_PERCENT=37.500
PRIMER_LEFT_19_SELF_ANY=3.00
PRIMER_RIGHT_19_SELF_ANY=3.00
PRIMER_INTERNAL_19_SELF_ANY=8.00
PRIMER_LEFT_19_SELF_END=0.00
PRIMER_RIGHT_19_SELF_END=0.00
PRIMER_INTERNAL_19_SELF_END=4.00
PRIMER_LEFT_19_END_STABILITY=6.4000
PRIMER_RIGHT_19_END_STABILITY=7.0000
PRIMER_PAIR_19_COMPL_ANY=6.00
PRIMER_PAIR_19_COMPL_END=3.00
PRIMER_PAIR_19_PRODUCT_SIZE=226
PRIMER_PAIR_20_PENALTY=3.830133
PRIMER_LEFT_20_PENALTY=1.806577
PRIMER_RIGHT_20_PENALTY=2.023556
PRIMER_INTERNAL_20_PENALTY=7.331111
PRIMER_LEFT_20_SEQUENCE=cgattctccccggtatcaa
PRIMER_RIGHT_20_SEQUENCE=aaagagaatgtggagggaacaa
PRIMER_INTERNAL_20_SEQUENCE=aacatgtcaactcttattctagccaac
PRIMER_LEFT_20=64,19
PRIMER_RIGHT_20=324,22
PRIMER_INTERNAL_20=269,27
PRIMER_LEFT_20_TM=60.807
PRIMER_RIGHT_20_TM=59.976
PRIMER_INTERNAL_20_TM=60.331
PRIMER_LEFT_20_GC_PERCENT=52.632
PRIMER_RIGHT_20_GC_PERCENT=40.909
PRIMER_INTERNAL_20_GC_PERCENT=37.037
PRIMER_LEFT_20_SELF_ANY=4.00
PRIMER_RIGHT_20_SELF_ANY=3.00
PRIMER_INTERNAL_20_SELF_ANY=6.00
PRIMER_LEFT_20_SELF_END=1.00
PRIMER_RIGHT_20_SELF_END=0.00
PRIMER_INTERNAL_20_SELF_END=0.00
PRIMER_LEFT_20_END_STABILITY=6.9000
PRIMER_RIGHT_20_END_STABILITY=7.0000
PRIMER_PAIR_20_COMPL_ANY=6.00
PRIMER_PAIR_20_COMPL_END=1.00
PRIMER_PAIR_20_PRODUCT_SIZE=261
PRIMER_PAIR_21_PENALTY=4.087459
PRIMER_LEFT_21_PENALTY=1.806597
PRIMER_RIGHT_21_PENALTY=2.280862
PRIMER_INTERNAL_21_PENALTY=2.515892
PRIMER_LEFT_21_SEQUENCE=agattcgattctccccggt
PRIMER_RIGHT_21_SEQUENCE=gacatgcatgattggatacgtt
PRIMER_INTERNAL_21_SEQUENCE=actttgttccctccacattctc
PRIMER_LEFT_21=59,19
PRIMER_RIGHT_21=356,22
PRIMER_INTERNAL_21=300,22
PRIMER_LEFT_21_TM=60.807
PRIMER_RIGHT_21_TM=59.719
PRIMER_INTERNAL_21_TM=59.484
PRIMER_LEFT_21_GC_PERCENT=52.632
PRIMER_RIGHT_21_GC_PERCENT=40.909
PRIMER_INTERNAL_21_GC_PERCENT=45.455
PRIMER_LEFT_21_SELF_ANY=8.00
PRIMER_RIGHT_21_SELF_ANY=8.00
PRIMER_INTERNAL_21_SELF_ANY=3.00
PRIMER_LEFT_21_SELF_END=2.00
PRIMER_RIGHT_21_SELF_END=2.00
PRIMER_INTERNAL_21_SELF_END=0.00
PRIMER_LEFT_21_END_STABILITY=11.1000
PRIMER_RIGHT_21_END_STABILITY=8.1000
PRIMER_PAIR_21_COMPL_ANY=3.00
PRIMER_PAIR_21_COMPL_END=0.00
PRIMER_PAIR_21_PRODUCT_SIZE=298
PRIMER_PAIR_22_PENALTY=4.106176
PRIMER_LEFT_22_PENALTY=0.744257
PRIMER_RIGHT_22_PENALTY=3.361920
PRIMER_INTERNAL_22_PENALTY=5.614815
PRIMER_LEFT_22_SEQUENCE=tgctttccttcatcagccat
PRIMER_RIGHT_22_SEQUENCE=cacccttttacacatttggattc
PRIMER_INTERNAL_22_SEQUENCE=tctcttcttgattcaatgaatctca
PRIMER_LEFT_22=511,20
PRIMER_RIGHT_22=654,23
PRIMER_INTERNAL_22=554,25
PRIMER_LEFT_22_TM=60.744
PRIMER_RIGHT_22_TM=59.638
PRIMER_INTERNAL_22_TM=59.385
PRIMER_LEFT_22_GC_PERCENT=45.000
PRIMER_RIGHT_22_GC_PERCENT=39.130
PRIMER_INTERNAL_22_GC_PERCENT=32.000
PRIMER_LEFT_22_SELF_ANY=3.00
PRIMER_RIGHT_22_SELF_ANY=4.00
PRIMER_INTERNAL_22_SELF_ANY=11.00
PRIMER_LEFT_22_SELF_END=2.00
PRIMER_RIGHT_22_SELF_END=3.00
PRIMER_INTERNAL_22_SELF_END=7.00
PRIMER_LEFT_22_END_STABILITY=9.6000
PRIMER_RIGHT_22_END_STABILITY=6.6000
PRIMER_PAIR_22_COMPL_ANY=3.00
PRIMER_PAIR_22_COMPL_END=0.00
PRIMER_PAIR_22_PRODUCT_SIZE=144
PRIMER_PAIR_23_PENALTY=4.194881
PRIMER_LEFT_23_PENALTY=2.367944
PRIMER_RIGHT_23_PENALTY=1.826937
PRIMER_INTERNAL_23_PENALTY=4.782051
PRIMER_LEFT_23_SEQUENCE=ctttgttccctccacattctc
PRIMER_RIGHT_23_SEQUENCE=gctgatgaaggaaagcaaga
PRIMER_INTERNAL_23_SEQUENCE=atcatgcatgtctgttctgatcat
PRIMER_LEFT_23=301,21
PRIMER_RIGHT_23=527,20
PRIMER_INTERNAL_23=345,24
PRIMER_LEFT_23_TM=58.632
PRIMER_RIGHT_23_TM=58.173
PRIMER_INTERNAL_23_TM=60.782
PRIMER_LEFT_23_GC_PERCENT=47.619
PRIMER_RIGHT_23_GC_PERCENT=45.000
PRIMER_INTERNAL_23_GC_PERCENT=37.500
PRIMER_LEFT_23_SELF_ANY=3.00
PRIMER_RIGHT_23_SELF_ANY=3.00
PRIMER_INTERNAL_23_SELF_ANY=8.00
PRIMER_LEFT_23_SELF_END=0.00
PRIMER_RIGHT_23_SELF_END=0.00
PRIMER_INTERNAL_23_SELF_END=4.00
PRIMER_LEFT_23_END_STABILITY=6.7000
PRIMER_RIGHT_23_END_STABILITY=7.0000
PRIMER_PAIR_23_COMPL_ANY=6.00
PRIMER_PAIR_23_COMPL_END=3.00
PRIMER_PAIR_23_PRODUCT_SIZE=227
PRIMER_PAIR_24_PENALTY=4.216158
PRIMER_LEFT_24_PENALTY=3.028128
PRIMER_RIGHT_24_PENALTY=1.188029
PRIMER_INTERNAL_24_PENALTY=5.389860
PRIMER_LEFT_24_SEQUENCE=ccaaatgtgtaaaagggtgtgat
PRIMER_RIGHT_24_SEQUENCE=atgggaacggaacgttgat
PRIMER_INTERNAL_24_SEQUENCE=tcaaagattgttcttaccaattcc
PRIMER_LEFT_24=636,23
PRIMER_RIGHT_24=836,19
PRIMER_INTERNAL_24=725,24
PRIMER_LEFT_24_TM=60.028
PRIMER_RIGHT_24_TM=60.188
PRIMER_INTERNAL_24_TM=58.610
PRIMER_LEFT_24_GC_PERCENT=39.130
PRIMER_RIGHT_24_GC_PERCENT=47.368
PRIMER_INTERNAL_24_GC_PERCENT=33.333
PRIMER_LEFT_24_SELF_ANY=4.00
PRIMER_RIGHT_24_SELF_ANY=6.00
PRIMER_INTERNAL_24_SELF_ANY=5.00
PRIMER_LEFT_24_SELF_END=2.00
PRIMER_RIGHT_24_SELF_END=2.00
PRIMER_INTERNAL_24_SELF_END=1.00
PRIMER_LEFT_24_END_STABILITY=6.3000
PRIMER_RIGHT_24_END_STABILITY=6.9000
PRIMER_PAIR_24_COMPL_ANY=3.00
PRIMER_PAIR_24_COMPL_END=2.00
PRIMER_PAIR_24_PRODUCT_SIZE=201
=
SEQUENCE_ID=Primers must end in AG
SEQUENCE_TEMPLATE=acaatattgtattggtgagatcatataagatttgatgtcaacatcttcgtaaaggtctcagattcgattctccccggtatcaatttaagtgagctaatttagcttcttaaaaaataaaatcaaacaacttttacataaactcagtgaaaacttggatataaagtatccttatactactctttagtcttgattagtctctgcaaagatatttatatgtactttgtattatcataagaacattcattgacattttaagttaatgaattactaacatgtcaactcttattctagccaacagttactttgttccctccacattctctttgaaatagtcaaacgtatccaatcatgcatgtctgttctgatcataacagcaaaagcatgtgtatagaaaattgatagttgaattagagtcattttccataaaaaaatattcaataagtgtgacattatttttcgtatgaattaatccattttttgctgatttgagattctttctttctttgcttcttgctttccttcatcagccattttttttgttttctctttctctctctcttcttgattcaatgaatctcaaaaatggattactattgttcattctgtttctggattgtgtttttttcaaagttgaatccaaatgtgtaaaagggtgtgatgtagctttagcttcctactatattataccatcaattcaactcagaaatatatcaaactttatgcaatcaaagattgttcttaccaattcctttgatgttataatgagctacaatagagacgtagtattcgataaatctggtcttatttcctatactagaatcaacgttccgttcccatgtgaatgtattggaggtgaatttctaggacatgtgtttgaatatacaacaaaagaaggagacgattatgatttaattgcaaatacttattacgcaagtttgacaactgttgagttattgaaaaagttcaacagctatgatccaaatcatatacctgttaaggctaag
PRIMER_PICK_RIGHT_PRIMER=1
PRIMER_PICK_INTERNAL_OLIGO=1
PRIMER_PICK_LEFT_PRIMER=1
PRIMER_NUM_RETURN=25
PRIMER_TASK=generic
PRIMER_EXPLAIN_FLAG=1
PRIMER_MIN_LEFT_THREE_PRIME_DISTANCE=1
PRIMER_MIN_RIGHT_THREE_PRIME_DISTANCE=1
PRIMER_MUST_MATCH_THREE_PRIME=nnnAG
PRIMER_MUST_MATCH_FIVE_PRIME=nnnnn
PRIMER_LEFT_EXPLAIN=considered 9032, GC content failed 12, low tm 301, high tm 19, high any compl 8, high end compl 11, long poly-x seq 2, failed must_match requirements 8638, ok 41
PRIMER_RIGHT_EXPLAIN=considered 9023, GC content failed 27, low tm 420, high tm 32, high any compl 2, high end compl 12, long poly-x seq 5, failed must_match requirements 8440, ok 85
PRIMER_INTERNAL_EXPLAIN=considered 9631, GC content failed 422, low tm 1156, high tm 51, long poly-x seq 37, failed must_match requirements 7722, ok 243
PRIMER_PAIR_EXPLAIN=considered 1708, unacceptable product size 1695, high end compl 3, no internal oligo 2, primer in pair overlaps a primer in a better pair 1352, ok 8
PRIMER_LEFT_NUM_RETURNED=8
PRIMER_RIGHT_NUM_RETURNED=8
PRIMER_INTERNAL_NUM_RETURNED=8
PRIMER_PAIR_NUM_RETURNED=8
PRIMER_PAIR_0_PENALTY=5.015480
PRIMER_LEFT_0_PENALTY=2.647536
PRIMER_RIGHT_0_PENALTY=2.367944
PRIMER_INTERNAL_0_PENALTY=7.331111
PRIMER_LEFT_0_SEQUENCE=ttctccccggtatcaatttaag
PRIMER_RIGHT_0_SEQUENCE=gagaatgtggagggaacaaag
PRIMER_INTERNAL_0_SEQUENCE=aacatgtcaactcttattctagccaac
PRIMER_LEFT_0=67,22
PRIMER_RIGHT_0=321,21
PRIMER_INTERNAL_0=269,27
PRIMER_LEFT_0_TM=59.352
PRIMER_RIGHT_0_TM=58.632
PRIMER_INTERNAL_0_TM=60.331
PRIMER_LEFT_0_GC_PERCENT=40.909
PRIMER_RIGHT_0_GC_PERCENT=47.619
PRIMER_INTERNAL_0_GC_PERCENT=37.037
PRIMER_LEFT_0_SELF_ANY=4.00
PRIMER_RIGHT_0_SELF_ANY=3.00
PRIMER_INTERNAL_0_SELF_ANY=6.00
PRIMER_LEFT_0_SELF_END=2.00
PRIMER_RIGHT_0_SELF_END=0.00
PRIMER_INTERNAL_0_SELF_END=0.00
PRIMER_LEFT_0_END_STABILITY=6.3000
PRIMER_RIGHT_0_END_STABILITY=7.3000
PRIMER_PAIR_0_COMPL_ANY=5.00
PRIMER_PAIR_0_COMPL_END=0.00
PRIMER_PAIR_0_PRODUCT_SIZE=255
PRIMER_PAIR_1_PENALTY=5.977824
PRIMER_LEFT_1_PENALTY=5.932992
PRIMER_RIGHT_1_PENALTY=0.044832
PRIMER_INTERNAL_1_PENALTY=2.515892
PRIMER_LEFT_1_SEQUENCE=tgtcaactcttattctagccaacag
PRIMER_RIGHT_1_SEQUENCE=ggctgatgaaggaaagcaag
PRIMER_INTERNAL_1_SEQUENCE=actttgttccctccacattctc
PRIMER_LEFT_1=273,25
PRIMER_RIGHT_1=528,20
PRIMER_INTERNAL_1=300,22
PRIMER_LEFT_1_TM=59.067
PRIMER_RIGHT_1_TM=59.955
PRIMER_INTERNAL_1_TM=59.484
PRIMER_LEFT_1_GC_PERCENT=40.000
PRIMER_RIGHT_1_GC_PERCENT=50.000
PRIMER_INTERNAL_1_GC_PERCENT=45.455
PRIMER_LEFT_1_SELF_ANY=4.00
PRIMER_RIGHT_1_SELF_ANY=3.00
PRIMER_INTERNAL_1_SELF_ANY=3.00
PRIMER_LEFT_1_SELF_END=2.00
PRIMER_RIGHT_1_SELF_END=0.00
PRIMER_INTERNAL_1_SELF_END=0.00
PRIMER_LEFT_1_END_STABILITY=6.7000
PRIMER_RIGHT_1_END_STABILITY=8.5000
PRIMER_PAIR_1_COMPL_ANY=5.00
PRIMER_PAIR_1_COMPL_END=1.00
PRIMER_PAIR_1_PRODUCT_SIZE=256
PRIMER_PAIR_2_PENALTY=8.162202
PRIMER_LEFT_2_PENALTY=2.314970
PRIMER_RIGHT_2_PENALTY=5.847232
PRIMER_INTERNAL_2_PENALTY=6.476933
PRIMER_LEFT_2_SEQUENCE=ttcccatgtgaatgtattggag
PRIMER_RIGHT_2_SEQUENCE=cagttgtcaaacttgcgtaataag
PRIMER_INTERNAL_2_SEQUENCE=acaacaaaagaaggagacgattat
PRIMER_LEFT_2=830,22
PRIMER_RIGHT_2=944,24
PRIMER_INTERNAL_2=881,24
PRIMER_LEFT_2_TM=59.685
PRIMER_RIGHT_2_TM=58.153
PRIMER_INTERNAL_2_TM=57.523
PRIMER_LEFT_2_GC_PERCENT=40.909
PRIMER_RIGHT_2_GC_PERCENT=37.500
PRIMER_INTERNAL_2_GC_PERCENT=33.333
PRIMER_LEFT_2_SELF_ANY=8.00
PRIMER_RIGHT_2_SELF_ANY=5.00
PRIMER_INTERNAL_2_SELF_ANY=3.00
PRIMER_LEFT_2_SELF_END=0.00
PRIMER_RIGHT_2_SELF_END=3.00
PRIMER_INTERNAL_2_SELF_END=3.00
PRIMER_LEFT_2_END_STABILITY=8.2000
PRIMER_RIGHT_2_END_STABILITY=5.9000
PRIMER_PAIR_2_COMPL_ANY=4.00
PRIMER_PAIR_2_COMPL_END=0.00
PRIMER_PAIR_2_PRODUCT_SIZE=115
PRIMER_PAIR_3_PENALTY=8.662949
PRIMER_LEFT_3_PENALTY=6.329720
PRIMER_RIGHT_3_PENALTY=2.333229
PRIMER_INTERNAL_3_PENALTY=5.707700
PRIMER_LEFT_3_SEQUENCE=tcataacagcaaaagcatgtgtatag
PRIMER_RIGHT_3_SEQUENCE=ggaaagcaagaagcaaagaaag
PRIMER_INTERNAL_3_SEQUENCE=tttttgctgatttgagattcttt
PRIMER_LEFT_3=365,26
PRIMER_RIGHT_3=518,22
PRIMER_INTERNAL_3=474,23
PRIMER_LEFT_3_TM=59.670
PRIMER_RIGHT_3_TM=59.667
PRIMER_INTERNAL_3_TM=57.292
PRIMER_LEFT_3_GC_PERCENT=34.615
PRIMER_RIGHT_3_GC_PERCENT=40.909
PRIMER_INTERNAL_3_GC_PERCENT=26.087
PRIMER_LEFT_3_SELF_ANY=4.00
PRIMER_RIGHT_3_SELF_ANY=2.00
PRIMER_INTERNAL_3_SELF_ANY=5.00
PRIMER_LEFT_3_SELF_END=2.00
PRIMER_RIGHT_3_SELF_END=0.00
PRIMER_INTERNAL_3_SELF_END=1.00
PRIMER_LEFT_3_END_STABILITY=4.9000
PRIMER_RIGHT_3_END_STABILITY=7.0000
PRIMER_PAIR_3_COMPL_ANY=2.00
PRIMER_PAIR_3_COMPL_END=0.00
PRIMER_PAIR_3_PRODUCT_SIZE=154
PRIMER_PAIR_4_PENALTY=10.238277
PRIMER_LEFT_4_PENALTY=4.180440
PRIMER_RIGHT_4_PENALTY=6.057837
PRIMER_INTERNAL_4_PENALTY=2.564187
PRIMER_LEFT_4_SEQUENCE=tcaacatcttcgtaaaggtctcag
PRIMER_RIGHT_4_SEQUENCE=ttgactatttcaaagagaatgtggag
PRIMER_INTERNAL_4_SEQUENCE=ttctccccggtatcaatttaa
PRIMER_LEFT_4=37,24
PRIMER_RIGHT_4=335,26
PRIMER_INTERNAL_4=67,21
PRIMER_LEFT_4_TM=59.820
PRIMER_RIGHT_4_TM=60.058
PRIMER_INTERNAL_4_TM=58.436
PRIMER_LEFT_4_GC_PERCENT=41.667
PRIMER_RIGHT_4_GC_PERCENT=34.615
PRIMER_INTERNAL_4_GC_PERCENT=38.095
PRIMER_LEFT_4_SELF_ANY=4.00
PRIMER_RIGHT_4_SELF_ANY=7.00
PRIMER_INTERNAL_4_SELF_ANY=4.00
PRIMER_LEFT_4_SELF_END=3.00
PRIMER_RIGHT_4_SELF_END=0.00
PRIMER_INTERNAL_4_SELF_END=4.00
PRIMER_LEFT_4_END_STABILITY=6.7000
PRIMER_RIGHT_4_END_STABILITY=8.2000
PRIMER_PAIR_4_COMPL_ANY=6.00
PRIMER_PAIR_4_COMPL_END=2.00
PRIMER_PAIR_4_PRODUCT_SIZE=299
PRIMER_PAIR_5_PENALTY=10.983089
PRIMER_LEFT_5_PENALTY=2.502561
PRIMER_RIGHT_5_PENALTY=8.480528
PRIMER_INTERNAL_5_PENALTY=5.614815
PRIMER_LEFT_5_SEQUENCE=gcttcttgctttccttcatcag
PRIMER_RIGHT_5_SEQUENCE=cattataacatcaaaggaattggtaag
PRIMER_INTERNAL_5_SEQUENCE=tctcttcttgattcaatgaatctca
PRIMER_LEFT_5=505,22
PRIMER_RIGHT_5=763,27
PRIMER_INTERNAL_5=554,25
PRIMER_LEFT_5_TM=60.503
PRIMER_RIGHT_5_TM=58.519
PRIMER_INTERNAL_5_TM=59.385
PRIMER_LEFT_5_GC_PERCENT=45.455
PRIMER_RIGHT_5_GC_PERCENT=29.630
PRIMER_INTERNAL_5_GC_PERCENT=32.000
PRIMER_LEFT_5_SELF_ANY=2.00
PRIMER_RIGHT_5_SELF_ANY=6.00
PRIMER_INTERNAL_5_SELF_ANY=11.00
PRIMER_LEFT_5_SELF_END=1.00
PRIMER_RIGHT_5_SELF_END=0.00
PRIMER_INTERNAL_5_SELF_END=7.00
PRIMER_LEFT_5_END_STABILITY=6.6000
PRIMER_RIGHT_5_END_STABILITY=5.7000
PRIMER_PAIR_5_COMPL_ANY=6.00
PRIMER_PAIR_5_COMPL_END=2.00
PRIMER_PAIR_5_PRODUCT_SIZE=259
PRIMER_PAIR_6_PENALTY=11.966876
PRIMER_LEFT_6_PENALTY=9.464315
PRIMER_RIGHT_6_PENALTY=2.502561
PRIMER_INTERNAL_6_PENALTY=2.515892
PRIMER_LEFT_6_SEQUENCE=cataagaacattcattgacattttaag
PRIMER_RIGHT_6_SEQUENCE=gctgatgaaggaaagcaagaag
PRIMER_INTERNAL_6_SEQUENCE=actttgttccctccacattctc
PRIMER_LEFT_6=229,27
PRIMER_RIGHT_6=527,22
PRIMER_INTERNAL_6=300,22
PRIMER_LEFT_6_TM=57.536
PRIMER_RIGHT_6_TM=60.503
PRIMER_INTERNAL_6_TM=59.484
PRIMER_LEFT_6_GC_PERCENT=25.926
PRIMER_RIGHT_6_GC_PERCENT=45.455
PRIMER_INTERNAL_6_GC_PERCENT=45.455
PRIMER_LEFT_6_SELF_ANY=7.00
PRIMER_RIGHT_6_SELF_ANY=3.00
PRIMER_INTERNAL_6_SELF_ANY=3.00
PRIMER_LEFT_6_SELF_END=3.00
PRIMER_RIGHT_6_SELF_END=0.00
PRIMER_INTERNAL_6_SELF_END=0.00
PRIMER_LEFT_6_END_STABILITY=6.3000
PRIMER_RIGHT_6_END_STABILITY=6.7000
PRIMER_PAIR_6_COMPL_ANY=5.00
PRIMER_PAIR_6_COMPL_END=0.00
PRIMER_PAIR_6_PRODUCT_SIZE=299
PRIMER_PAIR_7_PENALTY=13.686032
PRIMER_LEFT_7_PENALTY=5.629294
PRIMER_RIGHT_7_PENALTY=8.056737
PRIMER_INTERNAL_7_PENALTY=0.833045
PRIMER_LEFT_7_SEQUENCE=tttgatgtcaacatcttcgtaaag
PRIMER_RIGHT_7_SEQUENCE=ttgcagagactaatcaagactaaagag
PRIMER_INTERNAL_7_SEQUENCE=tctcagattcgattctcccc
PRIMER_LEFT_7=30,24
PRIMER_RIGHT_7=202,27
PRIMER_INTERNAL_7=55,20
PRIMER_LEFT_7_TM=58.371
PRIMER_RIGHT_7_TM=58.943
PRIMER_INTERNAL_7_TM=59.167
PRIMER_LEFT_7_GC_PERCENT=33.333
PRIMER_RIGHT_7_GC_PERCENT=37.037
PRIMER_INTERNAL_7_GC_PERCENT=50.000
PRIMER_LEFT_7_SELF_ANY=8.00
PRIMER_RIGHT_7_SELF_ANY=4.00
PRIMER_INTERNAL_7_SELF_ANY=8.00
PRIMER_LEFT_7_SELF_END=2.00
PRIMER_RIGHT_7_SELF_END=0.00
PRIMER_INTERNAL_7_SELF_END=0.00
PRIMER_LEFT_7_END_STABILITY=6.3000
PRIMER_RIGHT_7_END_STABILITY=6.7000
PRIMER_PAIR_7_COMPL_ANY=5.00
PRIMER_PAIR_7_COMPL_END=0.00
PRIMER_PAIR_7_PRODUCT_SIZE=173
=
